This study examined the mechanism by which cGMP contributes to the vasodilator response to nitric oxide (NO) in rat middle cerebral arteries (MCA). Administration of a NO donor, diethylaminodiazen-1-ium-1,2-dioate (DEA-NONOate), or 8-bromo-cGMP (8-BrcGMP) increased the diameter of serotonin-preconstricted MCA by 79 +/- 3%. The response to DEA-NONOate, but not 8-BrcGMP, was attenuated by iberiot...

Cawley SM, Kolodziej S, Ichinose F, Brouckaert P, Buys ES, Bloch KD. sGC 1 mediates the negative inotropic effects of NO in cardiac myocytes independent of changes in calcium handling. Am J Physiol Heart Circ Physiol 301: H157–H163, 2011. First published May 2, 2011; doi:10.1152/ajpheart.01273.2010.—In the heart, nitric oxide (NO) modulates contractile function; however, the mechanisms responsi...

The goal of this study was to test the hypothesis that the cardioprotective effects of the late phase of ischemic preconditioning (PC) can be mimicked by treatment with NO donors. In phase I (studies of myocardial stunning), conscious rabbits underwent a sequence of six 4-minute coronary occlusion/4-minute reperfusion cycles for 3 consecutive days (days 1, 2, and 3). In group I (controls, n=6),...

Kampf, C. 2001. Effects of Th-1 and Th-2 cytokines and reactive oxygen species on normal human bronchial epithelial cells. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1084. 64 pp. Uppsala. ISBN 91-554-5135-7. Epithelial damage and shedding of the epithelium are common observations in many airway diseases such as asthma, Sjögren’s...

Nitric oxide is produced during intestinal inflammation and inhibits the epithelial responsiveness to cAMP-dependent secretagogues. The effect is presumably due to inhibition of activation of the CFTR. However, because insertion of CFTR into the epithelial apical membrane is also a cAMP-dependent process, we tested the hypothesis that NO could inhibit cAMP-dependent CFTR trafficking. SCBN intes...

In the heart, nitric oxide (NO) modulates contractile function; however, the mechanisms responsible for this effect are incompletely understood. NO can elicit effects via a variety of mechanisms including S-nitrosylation and stimulation of cGMP synthesis by soluble guanylate cyclase (sGC). sGC is a heterodimer comprised of a β(1)- and an α(1)- or α(2)-subunit. sGCα(1)β(1) is the predominant iso...

An easy, effective and eco-friendly one-pot process for the preparation of 4H-pyran compounds has been established by using a catalytic amount reduced graphene oxide functionalized with diethylenetriamine (rGO-DETA) in water under mild conditions. The catalyst was very this reaction provided desired products 1a–t good to high yields (87%–97%) short times. Moreover, rGO-DETA composite could be r...