We previously described a novel tissue cryopreservation protocol to enable the safe preservation of various autologous stem cell sources. The present study characterized the stem cells derived from long-term cryopreserved dental pulp tissues (hDPSCs-cryo) and analyzed their differentiation into definitive endoderm (DE) and hepatocyte-like cells (HLCs) in vitro. Human dental pulp tissues from ex...

Objective:The aim of this studywas to characterize the structure of normal and inflammed pulpal connective tissue on samples of human dental pulp and recently extracted teeth. Materials andMethods:Morphological and histochemical stainingmethodswere used in order to reveal the particular cell types and fibers present in dental pulp, in normal and inflammatory conditions. Results: In the patholog...

INTRODUCTION Migratory cells via blood circulation or cells adjacent to the root apex may potentially participate in dental pulp tissue regeneration or renewal. This study investigated whether systemically transplanted bone marrow cells can contribute to pulp regeneration in a chimeric mouse model. METHODS A chimeric mouse model was created through the injection of bone marrow cells from gree...

Odontoblasts produce most of the extracellular matrix (ECM) components found in dentin and implicated in dentin mineralization. Major differences in the pulp ECM explain why pulp is normally a non-mineralized tissue. In vitro or in vivo, some dentin ECM molecules act as crystal nucleators and contribute to crystal growth, whereas others are mineralization inhibitors. After treatment of caries l...

Introduction Quantification of carious lesions and measurement of the distance to the pulp is an important task in conservative dentistry. This information can improve identification of cases with the potential for maintaining pulp vitality and monitor treatment success of indirect pulp capping. In the procedure of indirect pulp capping the nearly exposed pulp is covered with a chemical (usuall...

Transforming Growth Factor-betal (TGFB31) is a potent modulator of tissue repair in various tissues. To analyze its role during human dental repair, we used thick-sliced teeth cultured as described previously (Magloire et al., 1996). The supply of TGFB1 to the pulp tissue was accomplished by means of a small tube glued onto the dentin. We show that this device allowed the growth factor to diffu...

This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same mat...

In 6 dogs, experiments were performed to determine the tissue reaction of the dental pulp at the 7, 30 and 60 days after direct contact of the pulp with the obturation material through a microcommunication orifice with diameter up to 1 mm, formed in the fundic part of an experimental cavity. Capping of 36 mandibular premolars with traumatically exposed pulp was carried out. On the exposed pulp,...

Exogenous ideal delivery system for biologically active molecules is under intensive investigation. In the present study it was aimed to test the availability of different calcium phosphate biomaterials (CPBs), hydroxyapatite (HA), beta tricalcium phosphate (TCP), and a combination of these (TCP+HA) as dental pulp capping systems on healing of dog dental pulp. Experimentally perforated dental p...