OBJECTIVE:We evaluated the effectiveness of spermwashing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels us...

Density gradient ultracentrifugation of low density lipoproteins (LDL) from 12 normal subjects showed multiple, distinct isopycnic bands. Densitometric scanning of the gradient tubes revealed that each band could be assigned to one of four density intervals and that the boundaries of these intervals were consistent among all the subjects. Analytic ultracentrifuge flotation (S(f)(0)) rates were ...

OBJECTIVE To determine the effect of semen storage and separation techniques on sperm DNA fragmentation. DESIGN Controlled clinical study. SETTING An assisted reproductive technology laboratory. PATIENT(S) Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation. INTERVENTION(S) One aliquot from each sample was immediately prepared (control) for the ...

We report the preparation and characterization of poly(N-isopropylacrylamide) (PNIPAAm) polymer brushes exhibiting controlled lateral variations in the patchiness of polymer chains. These gradients were achieved through an atom transfer radical polymerization (ATRP) grafting-from approach utilizing surfaces on which the spatial profile of the initiator density was carefully controlled. Initiato...