Native chromatin IP assays were used to define changes in core histone acetylation at the lysozyme locus during developmental maturation of chicken macrophages and stimulation to high-level expression by lipo-polysaccharide. In pluripotent precursors the lysozyme gene (Lys) is inactive and there is no acetylation of core histones at the gene, its promoter or at the upstream cis-control elements...

Ku70 was originally described as an autoantigen, but it also functions as a DNA repair protein in the nucleus and as an antiapoptotic protein by binding to Bax in the cytoplasm, blocking Bax-mediated cell death. In neuroblastoma (NB) cells, Ku70's binding with Bax is regulated by Ku70 acetylation such that increasing Ku70 acetylation results in Bax release, triggering cell death. Although regul...

Androgen deprivation therapy (ADT) is a mainstay of prostate cancer treatment, given the dependence cells on androgen and receptor (AR). However, tumors become ADT-resistant, there need to understand mechanism. One possible mechanism upregulation AR co-regulators, although only handful have been definitively linked disease. We previously identified Mediator subunit MED19 as an co-regulator, rep...

Acetylation of multiple lysine residues in the p53 plays critical roles in the protein stability and transcriptional activity of p53. To better understand how p53 acetylation is regulated, we generated a number of p53 mutants and examined acetylation of each mutant in transfected cells. We found that p53 mutants that are defective in tetramer formation are also defective in C-terminal lysine re...

Lysine acetylation is a major posttranslational modification involved in a broad array of physiological functions. Here, we provide an organ-wide map of lysine acetylation sites from 16 rat tissues analyzed by high-resolution tandem mass spectrometry. We quantify 15,474 modification sites on 4,541 proteins and provide the data set as a web-based database. We demonstrate that lysine acetylation ...

the first zagreb index $m_1$ of a graph $g$ is equal to the sum of squaresof degrees of the vertices of $g$. goubko proved that for trees with $n_1$pendent vertices, $m_1 geq 9,n_1-16$. we show how this result can beextended to hold for any connected graph with cyclomatic number $gamma geq 0$.in addition, graphs with $n$ vertices, $n_1$ pendent vertices, cyclomaticnumber $gamma$, and minimal $m...

background : clinical work is important part of nursing teaching. nursing education to their responsibilities for teaching and researching are also responsible for integrating the knowledge and practice and offering service to community. complexity of these responsibilities often cause a great deal of psychological stress.   objective : to determine level of stress perceived by nurse educators ...

BACKGROUND N-acetyltransferase 2 (NAT2) is involved in both carcinogen detoxification through hepatic N-acetylation and carcinogen activation through local O-acetylation. NAT2 slow acetylation status is significantly associated with increased bladder cancer risk among European populations, but its association in Asian populations is inconclusive. METHODS NAT2 acetylation status was determined...

investigating transfer of one’s pragmatic knowledge of first language to his second or foreign language has been one of the areas of interest for researchers. however, there are contradictory results even within the scarce studies which have addressed transferability. this study was an attempt to investigate perceived pragmatic transferability of l1 refusal strategies by persian efl learners. s...

protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in saccharomyces cerevisiae and kluyveromyces marxianus. crude protein content of dried yeast cells were 53.22% and 45.6% for s.cerevisiae and k.marxianus, respectively. after 96 hrs of...