The lysis time of euglobulin clots made with whole blood (plasma and red cells) was very much shorter than that of clots made with plasma alone, indicating a fibrinolytic component in red cells. A plasminogen activator was found in the stroma-free hemolysate, and proteolytic activity was found in the stromal fraction. The plasminogen activator, purified by using diethylaminoethyl-cellulose (DEA...

We have determined that the organic matrix of calcium oxalate kidney stones contains a glycoprotein inhibitor of calcium oxalate crystal growth (nephrocalcin) that resembles nephrocalcin present in the urine of patients with calcium oxalate stones and differs from nephrocalcin from the urine of normal people. Pulverized calcium oxalate renal stones were extracted with 0.05M EDTA, pH 8.0; nephro...

An S-adenosyl-L-methionine:mRNA(nucleoside-2’-)methyltransferase, one of at least three activities required for the 5’-terminal modification of mRNA, has been purified from vaccinia virus particles. Employing brome mosaic virus RNA ending in m7G(5’)pppGas substrate, a simple DEAE-cellulose filter assay measuring the incorporation of methyl groups from S-adenosyl[methyL3H]methionine to position ...

Homogenates prepared from bovine heart muscle contain two distinct forms of malic enzyme (malate:NADP+ oxidoreductase (decarboxylating), EC 1.1.1.40), which can be easily separated by chromatography on DEAE-cellulose. A partial purflcation of the two enzymes has been accomplished by means of DEAE-cellulose chromatography, ammonium sulfate fractionation, and chromatography on CMSephadex. The iso...

The study of protein kinase in Dictyostelium discoideum has a tortuous history. The cyclic AMP-dependent protein kinase was first reported by Sampson [1]. Upon DEAE chromatography of cells starved for 2 h he found two cAMP-dependent and two cAMP-independent activities. Shortly thereafter reports by Veron and Patte [2] and Rahmsdorf and Gerisch [3] described protein kinase activity but no cAMP a...

Herpes simplex virus type I (HSV-1), infectious bovine rhinotracheitis virus (IBR) and turkey herpesvirus were examined for growth in cells cultured on three different substrates. The substrates were glass, DEAE-dextran and collagen gel. With two of the viruses, HSV-1 and IBR, there were no apparent differences in production as a function of substrate. In contrast, the amount of the turkey herp...

A deoxyribonuclease distinct from the previously isolated asparagus ribosome-inactivating proteins, possessing a molecular weight of 30 kDa and requiring a pH of 7.5 for optimum hydrolytic activity toward herring sperm DNA, was isolated from Asparagus officinalis seeds. The isolation procedure involved extraction with saline, (NH(4))(2)SO(4) precipitation, ion-exchange chromatography on DEAE-ce...

on either DEAE cellulose or Sephacryl S-200. About 80% of the reductase was one form in the chloroplast and the rest was a second form in the cytosol as determined by chromatography and by fractionation of subcellular organelles. The amount of activity detectable in the chloroplast fraction was 10.7 micromoles of dihydroxyacetone phosphate reductase per hour per milligram chlorophyll from spina...

The growth of transitional epithelial cells with different growth media and growth supports was examined. Sephadex G-10, Bio-Gel P-20, Bio-Glas-1000, DEAE-Sephadex A-50, DEAE-cellulose, CM-Sephadex C-50, acid-soluble collagen, and immobilized collagen fibers were used to enhance plating efficiency. Acid-soluble collagen layers optimally increased the plating efficiency of primary cultures of bl...

The human growth hormone (hGH) and human prolactin (hPRL) were iodinated with 125I using the lactoperoxidase method and purified by gel filtration on a Sephadex G-100 column and by ion exchange chromatography on a diethyl-aminoethyl (DEAE) cellulose column. Aliquots of the peak fraction on the Sephadex G-100 column and each fraction on the DEAE cellulose column were tested by radioimmunoassay (...