نتایج جستجو برای: de3 bl21

تعداد نتایج: 2244  

2012
Won-Heong Lee Panchalee Pathanibul Josh Quarterman Jung-Hyun Jo Nam Soo Han Michael J Miller Yong-Su Jin Jin-Ho Seo

BACKGROUND 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens. Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5'-diphosphate (GDP)-l-fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL producing Escherichia coli can be constructed through overexpressing genes codin...

Journal: :Research in Molecular Medicine 2021

Background: Interferon-gamma [IFN-γ) is the most important cytokine in immune system. This protein has been expressed bacterial cells. However, cloning not an easy task. We aimed to clone, express, and purify recombinant mouse IFN-γ overcome problems favor of commercial purposes. Materials Methods: To amplify gene product for cloning, we primarily designed two specific primers target gene. Foll...

Journal: : 2023

Mục tiêu: Xây dựng quy trình biểu hiện enzym tái tổ hợp Br512 và ứng dụng phát Human Papillomavirus type 18 bằng phương pháp khuếch đại đẳng nhiệt qua trung gian vòng lặp (LAMP). Vật liệu pháp: Plasmid pKAR2-Br512 mã hoá enzyme BR512 được biến nạp vào tế bào E.coli BL21(DE3). Enzyme từ đó chất cảm đặc hiệu (IPTG) sau tinh sạch sắc ký ái lực. Đánh giá tính của tự sản xuất, so sánh với Bst 2.0 (B...

Journal: :International journal of agriculture, environment and food sciences 2021

Gluten is a protein group found in wheat, barley, rye, and oats, known as cereals. When this vegetable introduced into the body, celiac disease can occurs. The use of bacterial fungal oligopeptidase to ensure cleavage gluten non-toxic fragments are considered promising alternative for disease. In study, Aspergillus niger Prolyl EndoPeptidase (AN-PEP) enzyme was cloned pET22b vector recombinantl...

فاکتور رشد عصبی(NGF) عضوی شناخته شده از خانواده ی پروتئین های نوروتروفین است که در درمان بیماری هایی مانند مولتی پل اسکلروزیس و آلزایمر بکار گرفته می شود. تولید پروتئین نوترکیب در E. coli دارای معایب خاصی است که شکل گیری اینکلوژن بادی و عدم شکل گیری پیوندهای دی سولفیدی درسیتوپلاسم در درجه اول قرار دارند .بنابراین ما به منظور هدایت β-NGF نوترکیب تولید شده به سمت پری پلاسم، استفاده از پپتید نشانه...

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