The self-replicating polerovirus-associated RNAs have recently been classified as tombusvirus-like associated (tlaRNAs). In a metagenomic comparison of the virome wild (Daucus carota subsp. carota) and cultivated (D. sativus) carrots, four tlaRNAs were identified: carrot red leaf virus-associated RNA (CtRLVaRNA), beet western yellows (BWYVaRNA), two other detected for first time in arracacha la...

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant–microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant-growth-promotion mechanisms of a Rhizobium leguminosarum strain named PEPV16 isolated from Ph...

Studies on the bioavailability of As, of impacted vegetables; and As bioaccesibility in the edible parts of carrots (daucus carota), beets (beta vulgaris) and quinoa (chenopodium) growing in polluted soils thought “in vitro” gastrointestinal process; the As speciation both in the edible parts of vegetables and in their gastrointestinal extracts have been performed. Elemental analysis and As spe...

Condensed mitotic chromosomes were isolated from cell suspension cultures of carrot, Daucus carota var. Danvers. Mitotic cells were obtained from partially synchronized cell cultures by adding colchicine 15 h after inoculation of 4-day-old cultures into fresh medium. From 12% to 18% of the cells were in mitosis 6 h after colchicine addition. Other methods of inducing synchrony and collecting mi...

The current work was investigated on the production of Invertase under solidstate fermentation (SSF) through Aspergillus species, by utilizing peels of Carrot (Daucus carota L.) as a substrate. The maximum production of Invertase (7.95±0.1 U/ml) was attained by using Aspergillus niger with 1×106 spores/ml at 90% moisture content after incubation period of 72 h. Through ammonium sulphate precipi...

Transfer of methotrexate and 5-methyltryptophan resistance from carrot (Daucus carota) to tobacco (Nicotiana tabacum) was achieved by fusion between leaf mesophyll protoplasts of tobacco and irradiated cell culture protoplasts of carrot. Some of the regenerated somatic hybrids exhibited normal tobacco morphology with coexpression and independent segregation of the transferred resistance markers...

The effect of proline analogs on peptidyl proline hydroxylation has been studied in vivo using aerated root slices of Daucus carota. One analog, 3,4-dehydroproline, acted at micromolar concentrations to rapidly and selectively inhibit peptidyl proline hydroxylation. A structurally altered hydroxyproline-rich cell wall glycoprotein was synthesized and secreted by dehydroproline-treated tissue. T...

Concerns about a global decline in pollinators have called for more knowledge the factors influencing wild pollinator abundance and diversity agroecosystems. Agricultural intensification has been identified as main cause of this “global crisis”, particularly due to reductions natural areas holding critical floral nesting resources. Maintaining native plants agricultural landscapes (e.g., field ...