UNLABELLED The Sec translocase pathway is the major route for protein transport across and into the cytoplasmic membrane of bacteria. Previous studies reported that the SecA translocase ATP-binding subunit and the cell surface HtrA protease/chaperone formed a single microdomain, termed "ExPortal," in some species of ellipsoidal (ovococcus) Gram-positive bacteria, including Streptococcus pyogene...

A Identifying peptides for their fragmentation spectra by database search sequencing method is crucial to interpret LC-MS/MS data, widely used algorithms had not been fully exploited the intensity patterns in fragment spectra, SQID incorporated intensity information and identified peptides significantly more peptides than Sequest and X!Tandem. Although SQID adopted various datasets which based ...

UNLABELLED Streptococcus pneumoniae is auxotrophic for arginine, and molecular analysis of the pneumococcal genome showed that the gene encoding an arginine-ornithine antiporter (ArcD) is organized in a cluster together with the arcABC genes encoding the arginine deiminase system (ADS) of pneumococci. The ADS consists of the arginine deiminase (AD), the catabolic ornithine carbamoyltransferase ...

UNLABELLED Streptococcus pneumoniae is an important commensal and pathogen responsible for almost a million deaths annually in children under five. The formation of biofilms by S. pneumoniae is important in nasopharyngeal colonization, pneumonia, and otitis media. Pneumolysin (Ply) is a toxin that contributes significantly to the virulence of S. pneumoniae and is an important candidate as a ser...

BACKGROUND Pleural infection affects about 65,000 patients annually in the US and UK. In this and other forms of pleural injury, mesothelial cells (PMCs) undergo a process called mesothelial (Meso) mesenchymal transition (MT), by which PMCs acquire a profibrogenic phenotype with increased expression of α-smooth muscle actin (α-SMA) and matrix proteins. MesoMT thereby contributes to pleural orga...

Streptococcus pneumoniae persist in the human nasopharynx within organized biofilms. However, expansion to other tissues may cause severe infections such as pneumonia, otitis media, bacteremia, and meningitis, especially in children and the elderly. Bacteria within biofilms possess increased tolerance to antibiotics and are able to resist host defense systems. Bacteria within biofilms exhibit d...

T he environmental issues have received a lot of attention in recent years. Since environmental pollution is one of the issues that may damage the ability of future generations, it is important to examine the determinants of the environment quality. The main objective of this study is to investigate the impact of urban and rural income inequality on natural gas consumption in Iran. To this...

•Binding analysis utilizing computational, biochemical, and structural techniques•PotF binds several compounds from polyamine biosynthesis•Crystal structures of closed open apo states emphasize PotF’s plasticity•Binding pocket hydration pattern affects thermodynamic profile for the ligands Periplasmic binding proteins (PBPs) are ubiquitous receptors in gram-negative bacteria. They sense solutes...

Scab, caused by Cladosporium cucumerinum Ell. et Arthur, is a prevalent disease of cucumber (Cucumis sativus L.) worldwide. Scab can cause serious losses for cucumber production, especially in protected culture such as high tunnel production. Resistance to cucumber scab is dominant and is controlled by a single gene, Ccu. Breeding for resistant cultivars is the most efficient way to control the...