OBJECTIVES The potential applications and industrial production of multi-wall carbon nanotubes (MWCNT) have raised serious concerns about their safety for human health and the environment. The present study was designed to examine the in vitro cytotoxicity and genotoxicity of MWCNT and UICC chrysotile A (chrysotile). METHODS Cytotoxicity using both colony formation and lactate dehydrogenase (...

Abstract Objectives: We have shown that chimeric antigen receptor (CAR) T cells with T-cell intrinsic PD1 dominant negative (PD1DNR) prolong functional persistence. Herein, we further define and extrinsic mechanistic functions of PD1DNR. Methods: Human were retrovirally transduced mesothelin targeted, CD28 co-stimulated CARs– either M28z (M) or PD1DNR (MDNR). Cytotoxicity, proliferation, phenot...

1.0 STANDARD OPERATING PROCEDURE (SOP) FOR THE BALB/C 3T3 NEUTRAL RED UPTAKE CYTOTOXICITY TEST A TEST FOR BASAL CYTOTOXICITY....................... 3 1.1 Background and Introduction.................................................................................................. 3 1.2 Rationale.........................................................................................................

T-2 toxin is a secondary fungal metabolite produced by various species of Fusarium. It is capable of killing cells by causing extensive damage to the cellular membrane. In this study, cytotoxicity of T-2 toxin in combination with different antioxidant materials, including vitamin C (vit. C), vitamin E (vit. E) and selenium (sel) was investigated in vitro using the neutral red cytotoxicity assay...

Thermostable direct hemolysin (TDH), a major virulence factor of Vibrio parahaemolyticus, induces cytotoxicity in cultured cells. However, the mechanism of TDH's cytotoxic effect including its target molecules on the plasma membrane of eukaryotic cells remains unclear. In this study, we identified the role of lipid rafts, cholesterol- and sphingolipid-enriched microdomains, in TDH cytotoxicity....

INTRODUCTION The aim of this in vitro study was to evaluate the cytotoxicity of a new nano zinc-oxide eugenol (NZOE) sealer on human gingival fibroblasts (HGFs) compared with Pulpdent (micro-sized ZOE sealer) and AH-26 (resin-based sealer). METHODS AND MATERIALS The Pulpdent, AH-26, and NZOE sealers were prepared and exposed to cell culture media immediately after setting, and 24 h and one we...

Rat 3Y1 fibroblasts transformed by the E1A gene of adenovirus type 12 (E1A-3Y1 cells) are highly sensitive to the cell-killing effect of 1,3-dilinoleoylglycerol (DLG) administered in a culture medium, whereas the parental 3Y1 cells are less sensitive (H. Shimura et al., Cancer Res., 48: 578-583, 1988). The selective cytotoxicity of DLG to E1A-3Y1 cells was markedly reduced by the simultaneous a...

UNLABELLED Cell culture system has been used to evaluate alloy cytotoxicity under different environments, testing the extracts, but the effect of temperature variation on the cytotoxicity of dental alloys has not been analyzed. OBJECTIVE The aim of the present study was to investigate if temperature variation could affect dental alloy cytotoxicity, testing alloy extracts in an epithelial cell...