1. The effect of disulfiram on the activity of the cytoplasmic and mitochondrial aldehyde dehydrogenases of sheep liver was studied. 2. Disulfiram causes an immediate inhibition of the enzyme reaction. The effect on the cytoplasmic enzyme is much greater than on the mitochondrial enzyme. 3. In both cases, the initial partial inhibition is followed by a gradual irreversible loss of activity. 4. ...

Most mammalian cells maintain a physiological cytoplasmic pH of approximately 7.2 despite the prediction of a more acidic internal pH on the basis of thermodynamic and metabolic considerations. If the transmembrane gradient of hydrogen ions were determined solely by a hydrogen ion leak pathway in the presence of a membrane potential of -60 mV, then the cytoplasmic pH would be about 6.2, that is...

Interactions between protein domains and lipid molecules play key roles in controlling cell membrane signalling and trafficking. The pleckstrin homology (PH) domain is one of the most widespread, binding specifically to phosphatidylinositol phosphates (PIPs) in cell membranes. PH domains must locate specific PIPs in the presence of a background of approximately 20% anionic lipids within the cyt...