The plate-dilution frequency technique described facilitates simultaneous enumeration of a wide range of physiologically different microorganisms in complex systems with a precision comparable to dilution tube (most probable number) methods. Replicate microsamples are inoculated from each member of a dilution series onto areas delineated on plates of prepoured solid media; the plates are incuba...

Background & Aim:Belongs to Rosaecea family, Rosa foetida is one the Persian native plants which has not been investigated biologically. As it is traditionally used topically as poultice to treat infectious skin burns, the present paper focused on the assessment of the antibacterial activities of different extracts of R. foetida flowers against the main cause of skin burn woun...

A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for...

A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby...

A plate assay which utilizes the addition of a blood agar overlay for detection of hemolysin by colonies of Leptospira interrogans serovar pomona is described.

introduction: glycyrrhiza glabra (licorice) root, a member of the pea family has been used since ancient times as both food and medicine. licorice has been used as an antioxidant, antimicrobial and anti inflammatory in researches .extraction of this medical plant is used as the basis of anti-ulcer medicine for treatment of peptic ulcer. patients and methods: in the present study, licorice decoc...

There is a growing interest in food industry to replace the synthetic chemicals by the natural products with bioactive properties from plant origin. The aim of this study was to determine the chemical composition of Mentha pulegium essential oil and to characterize the antimicrobial activities of the essential oil. The essential oil of Mentha pulegium was analyzed by GC-MS. The evaluation of th...

OBJECTIVES This study was undertaken to isolate a fibrinolytic enzyme from the snake venom of Gloydius blomhoffii siniticus and to investigate its enzymatic characteristics and hemorrhagic activity as a potential pharmacopuncture agent. METHODS The fibrinolytic enzyme was isolated by using chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and fibrin plate assay. The c...