by using special staining reagents on TLCshould result in interesting precursors for alkaloid synthesis. The screening was performed by centrifugation of the culture-broth from different Streptomyces strains, adsorption of the compounds in the culture filtrate on Amberlite XAD-16, elution with methanol and concentration. Detection of the amines after TLC separation of the products was done usin...

This is measured by a Boyden diffusion chamber. A filter separates cells, that can be labelled with radiochromium, and the attractant. Hence the number of neutrophils that move through the filter can be quantitated (Boyden, 1962; Ternowitz, 1985). One can also use a test in which there is migration of neutrophils under an agarose gel. Three wells are cut in agarose gel in a plastic tissue cultu...

The antagonistic activity of three bacterial isolates namely Microbacterium terregens, Cellulosimicrobium cellulans and Bacillus amylollquefaciens was evaluated through direct confrontation method and filtrates culture against the growth of Fusarium solani, Fusarium oxysporum, Rhizoctonia solani and Phytophthra cactorum. All bacterial isolates showed the inhibition of the mycelia growth of the ...

Biochemical variability among 20 Indian isolates of Sclerotinia sclerotiorum collected from different hosts/soil samples from different localities in India is reported. High Performance Liquid Chromatographic (HPLC) analysis of ethyl acetate fraction of culture filtrate, mycelia, sclerotia and sclerotial exudate showed 15~23 peaks but only 11 could be identified. They were tannic, gallic, oxali...

Cordycepin was first isolated from the culture filtrate of the mold Cordyceps militaris by Cunningham et al. (1) and subsequently characterized by Bentley, Cunningham and Spring (2) as 3’-deoxyadenosine with a branched chain. Kaczka et al. (3) have recently isolated from the filtrate of Aspergillus nidulans a material inhibiting the growth of KB cells and identified the active component as 3’-d...

In previous communications (1-8) there was described a phenomenon of local skin reactivity to culture filtrates of bacillus typhosus and other microorgamsms. The reactivity was induced by the injection of a filtrate into the skin of a rabbit. If 24 hours later an intravenous injection of the same filtrate was given to the rabbit, there appeared an extremely severe hemorrhagic necrosis at the si...

Arthrobacter sp. 10137 has been used to produce b-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the crude FFase, obtained by ultrafiltration and (NH4)2SO4 fractionation, was purified about 7-fold ...

The effect of antifungal compound from Bacillus subtilis strain LB5 was tested against conidial germination of Colletotrichum gloeosporioides and Pestalotiopsis eugeniae, causal agent of anthracnose and fruit rot of wax apple, respectively. Observation under scanning electron microscope and light compound microscope revealed that conidial germination was completely inhibited when treated with c...

A membrane filtration technique with commercially available membrane filters (Millipore Corp.) was effective for the removal of Reiter treponemes from liquids such as fluorescent-antibody conjugates, to which the organisms are added for adsorption. Reiter treponemes from an 8-day culture were not microscopically detectable in filtrates through membranes with a pore diameter of 0.45 mum, but tre...