PURPOSE To evaluate the effect of prolonged limbal explants cultured without any scaffolds or on amniotic membrane (AM) on the viability, proliferation and differentiation potential of putative phenotypically defined cultured limbal mesenchymal (LMSC) and epithelial stem cells (LESC). METHODS Limbal explants were cultivated on cryopreserved intact AM or plastic plates using medium supplemente...

KAMLER JIŘÍ, HOMOLKA MILOSLAV. 2016. The Importance of Cultivated Plants in the Diet of Red and Roe Deer and Moufl on. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(3): 813–819. The botanical composition of red and roe deer and moufl on diet was studied in the mosaic landscape in Drahanská vrchovina highlands, Czech Republic. We focused on the proportion and quality...

We developed 23 polymorphic microsatellite markers for the symbiotic fungi cultivated by leaf cutter ants, then assessed allelic variation in North American leafcutter-fungus populations (Mexico, Cuba, USA). Polyploidy was indicated by 21 of the 23 loci, consistent with the multinucleate nature of leafcutter fungi. Microsatellite fingerprinting can now assess fungal genetic variation within lea...

Flavobacterium spp. have been cultivated from diverse aquatic and terrestrial habitats. F. akiainvivens IK-1(T) was cultivated from decaying wood of Wikstroemia oahuensis, an endemic Hawaiian shrub. The strain's genome sequence may provide insights into niche adaptation and evolution of the genus in a mid-ocean archipelago.

RATIONALE It is important for the enforcement of the CITES treaty to determine whether agarwood (a resinous wood produced in Aquilaria and Gyrinops species) seen in trade is from a plantation that was cultivated for sustainable production or was harvested from natural forests which is usually done illegally. METHODS We analyzed wood directly using Direct Analysis in Real Time (DART™) ionizati...

PURPOSE To compare the in vitro antiangiogenic activities of ex vivo expanded human limbocorneal epithelial (HLE) cells cultivated on preserved human amniotic membrane (AM) and to identify factors responsible for the activities. METHODS The antiangiogenic effects were compared of culture media conditioned by AM, HLE cells, or HLE cells cultivated on intact AM (HLE/IAM), on denuded AM (HLE/DAM...

Phytoremediation is a green technology; however, very few species of arid environments have been identified as hyperaccumulators and fast growers. Therefore, greenhouse experiment was performed to evidence the phytoaccumulation potential Conocarpus erectus, Syzygium cumini, Populus deltoides Morus alba at sapling stage. Six-month-old plant saplings were subjected control (CK; 0 µM) cadmium trea...

Climate is a driver of terrestrial ecosystem carbon exchange, which is an important product of ecosystem function. The Qinghai-Tibetan Plateau has recently been subjected to a marked increase in temperature as a consequence of global warming. To explore the effects of warming on carbon exchange in grassland ecosystems, we conducted a whole-year warming experiment between 2012 and 2014 using ope...