the internal structure of dna-ctab and dna-ddab is investigated by a small angel x- ray scattering(saxs) instrument. hexagonal packing of dna was observed for dna complex with ctab, and for ddabcomplex is observed lamellar structure. variations in the internal spacing and degree of long-range ordering aredependent on both surfactant type and concentrations of added salt. when we increased the a...

Amplification inhibitors can lead to false-negative results for PCR. In order to evaluate the reliability of PCR for the detection of Chlamydia pneumoniae, the presence of PCR inhibitors in 75 bronchoalveolar lavage specimens was assessed after treatment by various sample preparation methods. Specimens were collected from patients with acute respiratory infections, including four cases of prove...

The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free fr...

The aim of this study was to develop a microemulsion system as a medium for laccase-catalyzed reactions. Phase behavior studies were conducted by constructing partial pseudo-ternary phase diagrams for systems comprising of cetyltrimethylammonium bromide (CTAB), various organic solvents as the oil phase (i.e., hexane, cyclohexane, heptane, octane, isooctane, toluene, isopropyl myristate), two co...

In Present work, the main objective is to develop less time consuming protocol for genomic DNA isolation from leaves of Passiflora foetida. Optimized protocol is cost effective, as it avoided use of expensive liquid nitrogen. The important parameters of CTAB buffer composition such as Polyvinylpyrrolidone PVP40000 (without PVP, 1%, 2%, 3.5%, 4.0%, 4.5%, 5.0%), CTAB (w, 1%, 2%, 3%, 4%, 5%), wate...

Uniformly sized and shape-controlled nanoparticles are important due to their applications in catalysis, electrochemistry, ion exchange, molecular adsorption, and electronics. Several ferric phosphate hydroxide (Fe4(OH)3(PO4)3) microstructures were successfully prepared under hydrothermal conditions. Using controlled variations in the reaction conditions, such as reaction time, temperature, and...

In this paper, application of sawdust prepared from Narra wood (SD) modified with cationic surfactant of cetyltrimethylammonium bromide (SD/CTAB) was used for removal of two typical acid dyes commercially named as Acid Green 25 (AG25) and Acid Red 14 (AR14) from aqueous solutions. The effects of experimental variables, such as pH, initial dye concentration, adsorbent dose, contact time and temp...

Abstract BACKGROUND Non-small cell lung cancer (NSCLC) accounts for ~50% of brain metastases. We present the genomic and immune biomarker landscape a cohort with metastatic NSCLC to brain. METHODS analyzed metastases FFPE tissue (n=137; ages 40-85y (mean 65y), 52% female, 48% male) vs. primary sites (n=5533; 24-100+y 71y), 51% 49% advanced or patients comprehensive profiling, including PD-L1 IH...

Compact MnCoS thin films on a nickel foam (NF) substrate were prepared by successive ionic layer adsorption and reaction (SILAR) method, two surfactants (SDS CTAB) used to improve the wettability of NF. The characterized scanning electron microscopy (SEM), transmission (TEM), X-ray diffraction (XRD), photoelectron spectroscopy (XPS). supercapacitive properties evaluated cyclic voltammetry (CV),...

The assembly of colloidal hyaluronic acid (HyA, as a polysaccharide) based hydrogel particles in an aqueous medium is characterized the present paper, with emphasis on particular case nanohydrogels formed by surfactant-neutralized polysaccharide networks. structural changes and particle formation process polysaccharide- cationic-surfactant-containing systems were induced charge neutralization a...