the aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2seal and ah plus, in two osteoblast-like cell lines, mg-63 and saos-2. using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. the extracts were exchanged with saos-2 or mg-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability t...

objective(s):sea cucumber is one of the classes of echinoderms, which is considered as a health marine product and possess various biological characteristics with therapeutic application. the present investigation attempted to evaluate the potential of anti-cancer persian gulf sea cucumber species holothuria arenicola (h. arenicola) aqueous extract on mice colon carcinoma cells in vitro and in ...

Background and Objectives. To determine the most appropriate technique for tumour followup in experimental therapeutics, we compared ultrasound (US) and magnetic resonance imaging (MRI) to characterize ectopic and orthotopic colon carcinoma models. Methods. CT26 tumours were implanted subcutaneously (s.c.) in Balb/c mice for the ectopic model or into the caecum for the orthotopic model. Tumours...

This study investigated microcirculatory-blood-flow responses in nude mice following the injection of CT26 tumor cells by analyzing the frequency content of skin blood-flow signals recorded on the skin surface. CT26 cells were injected subcutaneously (10^4/100 μl) into the right back flank of each 7-week-old mouse. Three-minute laser Doppler flowmetry (LDF) signals were measured in 60 nude mice...

OBJECTIVES The current study was designed to test the hypothesis that inhibition of poly(ADP-ribose) polymerase in colorectal cancer mediates down-regulation of metastasis-related gene expression through the regulation of nuclear factor-kappaB (NF-kappaB) activity. METHODS Mouse colon carcinoma cells (CT26) were treated with and without the PARP inhibitor 5-aminoisoquinolin-1(2H)-one hydrochl...

7-Ethyl-10-hydroxycamptothecin (SN38), an active metabolite of irinotecan (CPT-11), is a remarkably potent antitumor agent. The clinical application of SN38 has been extremely restricted by its insolubility in water. In this study, we successfully synthesized two macromolecular prodrugs of SN38 with different conjugate positions (chitosan-(C10-OH)SN38 and chitosan-(C20-OH)SN38) to improve the w...

objective: bone marrow mesenchymal stem cells reside in bone marrow and support homing and differentiation of hematopoietic stem cells (1). this makes them an excellent instrument for regenerative treatment and co-culture with the hsc. materials and methods: in this study, k562 cell lines were treated with butyric acid(for erythroid differentiation), co-cultured with mesenchymal stem cells and ...