3D Cryo-Bioprinting A vertical cryo-bioprinting technique is reported by Yu Shrike Zhang and co-workers in article number 2108931, which concurrently allows the user to create freestanding filamentous structures containing interconnected, anisotropic microchannels featuring gradient sizes aligned direction, also associated with enhanced mechanical performance. This method further extended a mul...

In this study the cryo-unit is introduced as a new and useful instrument to investigate water-containing specimens with the SEM. Often water-containing biological specimens are studied, but in our case we used water-swollen polymer membranes. The results show that application of a cryo-unit permits the study of this material at low temperatures up to magnifications of about 10 000 times, while ...

Nano fillers are part of new studies to enhance various properties of fluids and solids. For example, adding nanoparticles as an enforcement in nanocomposites or as an additive to improve thermal or electrical properties in nano-fluids are of extreme importance in science and industry. There are numerous methods to uniformly disperse nanoparticles in fluid and solid phase. One of the well-used ...

Abstract Objective: In vitro cytokine production by peripheral blood mononuclear cells (PBMCs) is an important and reliable measure of immunocompetence. PBMC can be stimulated directly after isolation or frozen for later use. However, cryopreservation may affect cell recovery, viability functionality. This study aims to investigate synthesis in ex-vivo fresh cryopreserved CD4+ CD4- T cells. Met...

Abstract Funding Acknowledgements Type of funding sources: None. Introduction – Catheter ablation (CA) is a first-choice treatment for tachyarrhythmia in pediatric patients. The currently available CA techniques differ manner catheter steering technique and energy sources. There are no large studies comparing long-term outcomes between the patients with atrioventricular reentry (also known as a...

As part of its remit to provide computational support to the cryo-EM community, the Collaborative Computational Project for Electron cryo-Microscopy (CCP-EM) has produced a software framework which enables easy access to a range of programs and utilities. The resulting software suite incorporates contributions from different collaborators by encapsulating them in Python task wrappers, which are...

Cryo-electron tomography (cryo-ET) captures the 3D electron density distribution of macromolecular complexes in close to native state. With the rapid advance of cryo-ET acquisition technologies, it is possible to generate large numbers (>100,000) of subtomograms, each containing a macromolecular complex. Often, these subtomograms represent a heterogeneous sample due to variations in the structu...

In response to events such as receptor binding and endocytic triggers, viruses undergo largescale, dynamic conformational changes necessary for cell entry and genome delivery. In later stages of the infectious cycle, replication machinery must read and synthesize nucleic acid strands to generate new copies of genetic material, and structural proteins must assemble and package the appropriate co...

Understanding the function of cellular machines requires a thorough analysis of the structural elements that underline their function. Electron microscopy (EM) has been pivotal in providing information about cellular ultrastructure, as well as macromolecular organization. Biological materials can be physically fixed by vitrification and imaged with cryo-electron tomography (cryo-ET) in a close-...