Most site-specific recombinases can be grouped into two structurally and mechanistically different classes. Whereas recombination by tyrosine recombinases proceeds with little movements by the proteins, serine recombinases exchange DNA strands by a mechanism requiring large quaternary rearrangements. Here we use site-directed crosslinking to investigate the conformational changes that accompany...

PURPOSE We report a case of acute corneal melt with perforation in a patient with keratoconus after collagen crosslinking treatment and the use of topical diclofenac and proparacaine eyedrops. METHODS The authors present a case report with clinicopathologic correlation. RESULTS A patient diagnosed with keratoconus underwent corneal collagen crosslinking followed by postoperative use of oflo...

Formaldehyde Crosslinking. Cells (10) suspended in growth medium are transferred as 40-ml aliquots into 50-ml tubes and placed on ice for 10 min. Then 1/10 volume of crosslinking solution (11% formaldehyde/0.1M NaCl/1mM Na-EDTA/0.5 mM NaEGTA/50mM Hepes, pH 8.0) is added directly to each tube. After 10-min incubation on ice, 1/20 vol of a 2.5-M glycine solution is added to each tube to stop the ...

In the present work, 3D-printed wound dressings containing different amounts of Centella Asiatica extract were synthesized via dual-crosslinking method. Methacrylic anhydride modified gelatin (Gel-MA) and pectin (Pec) chosen as base material for dressings. A dual crosslinked network was formed with Gel-MA photo-crosslinking using Irgacure 2959 Pectin psychical-crosslinking Ca++ ions. Meanwhile,...

Elastomers or rubbers are materials used in a large range of industrial and household applications. A common physical-chemical treatment is curing (crosslinking), imparting the rubber mechanical and thermal stability. Elastomers show low thermal conductivity values, and therefore, require complex and high cost heating methods; thus, the ionizing (accelerated electrons) method shows high interes...

Abstract: This paper reports the reaction of cotton cellulose with sulphamic acid using pad bake process. FT-IR and FT-Raman were used to analyse the intermediate products formed during this study. The self-crosslinking of the modified cotton fibre has special characteristics including good dye ability in case of ammonia or ethanolamine initiated crosslinking. Peroxide should react with the mod...

Poly (vinyl alcohol) membranes were prepared by in-situ crosslinking of poly (vinyl alcohol) with glutaraldehyde as a crosslinking agent and hydrochloric acid as catalyst and the membranes were used to separate mixtures of IPA and water. Effects of feed composition, operating temperature, vacuum pressure and Reynolds number on permeation performance of the membranes were evaluated. The results ...