The main purpose of this study is developed and drug loaded nanoparticles of azelastine hydrochloride, an antiallergic drug and optimization in terms of chemical properties, drug concentration, polymer concentration, cross‐linking agent and stirring time. Nanoparticles of azelastine hydrochloride were fabricated using chitosan and sodium alginate as polymers by ionotropic pregelation method. Ca...

Glycosaminoglycans, the most abundant heteropolysaccharides, have an inevitable role in tissue hydration, cell adhesion, regulation of cell growth and proliferation. Glycosaminoglycanchitosan-gelatin (GCG) scaffold incorporated with Hemigraphis colorata silver nanoparticles was prepared. The study confirmed the physical cross linking of the scaffold using scanning electron microscopy (SEM) and ...

The concept of crosslinking involves a complicated process of formation of chemical bonds between larger molecules that can change the structure of the material, make it stronger and more resistant to decay. Physical crosslinking with ultraviolet light is used in various fields of industry and medicine for increasing the strength of the material. Chemical crosslinking methods are used in stomat...

Most infants with long-gap esophageal atresia receive an esophageal replacement with tissue from stomach or colon, because the native esophagus is too short for true primary repair. Tissue-engineered esophageal conducts could present an attractive alternative. In this paper, circular decellularized porcine esophageal scaffold tissues were implanted subcutaneously into Sprague-Dawley rats. Depen...

pH-sensitive poly(acrylic acid) (PAA) hydrogel reinforced with cellulose nanocrystals (CNC) was prepared. Acrylic acid (AA) was subjected to chemical cross-linking using the cross-linking agent MBA (N,N-methylenebisacrylamide) with CNC entrapped in the PAA matrix. The quantity of CNC was varied between 0, 5, 10, 15, 20, and 25 wt %. X-ray diffraction (XRD) data showed an increase in crystallini...

Stabilization of multimeric enzymes is one of the major challenges in biocatalysis, because dissociation of subunits can inactivate the enzyme. Catalase (CAT; EC 1.11.16) is a homotetramer containing Fe-protoporphyrin IX in its active site. CAT breaks down hydrogen peroxide into water and molecular oxygen. In this study, crosslinked enzyme aggregates of bovine liver CAT (CAT-CLEAs) were prepare...

A separable and highly-stable enzyme system was developed by adsorption of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis in amino-functionalized macroporous silica gel and subsequent enzyme crosslinking. This resulted in the formation of cross-linked enzyme aggregates (PAL-CLEAs) into macroporous silica gel (MSG-CLEAs). The effect of adsorptive conditions, type of aggregating agen...

Described herein is interfacial bioorthogonal cross-linking, the use of bioorthogonal chemistry to create and pattern biomaterials through diffusion-controlled gelation at the liquid-gel interface. The basis is a rapid (k2 284000 M-1 s-1) reaction between strained trans-cyclooctene (TCO) and tetrazine (Tz) derivatives. Syringe delivery of Tz-functionalized hyaluronic acid (HA-Tz) to a bath of b...

There has been substantial recent interest in aligning mentions of named-entities in unstructured texts to knowledge base descriptors, a task commonly called entity linking. This technology is crucial for applications in knowledge discovery and text data mining. This paper presents experiments in the new problem of crosslanguage entity linking, where documents and named entities are in a differ...