The near-neighbor relationships of proteins in the myelin membrane were examined using dinitrodifluorobenzene and other cross-linking reagents. When intact cat dorsal column or isolated myelin fragments were treated with cross-linking reagents, up to 20% of the myelin basic protein dimerized. The only other cross-linked product formed in the intact cat dorsal column was a heterodimer consisting...

background: enzyme linked immunosorbent assay (elisa) has been described as an alternative to radioimmunoassay for the mammalian and nonmammalian steroids detection. in this study, a simple and rapid elisa is described and validated for 4-pregnen-3,20, dione (progesterone). materials and methods: a general procedure for preparation of the acetylcholinesterase labelled steroid is described which...

Glutaraldehyde is well known for its ability to react with proteins and to produce insoluble cross-linked aggregates. In contrast with this situation, conditions are described here which yield covalently linked soluble protein oligomers. The procedure is applicable to a wide range of proteins, and by slight variation in the reaction conditions, soluble polymers in the molecular weight range 3x1...

The interaction of asparagine-linked carbohydrates (N-linked) with carbohydrate binding proteins called lectins has been demonstrated to be involved in a variety of cellular recognition processes. Certain N-linked carbohydrates have been shown to be multivalent and capable of binding, cross-linking, and precipitating lectins (Bhattacharyya, L., Ceccarini, C., Lorenzoni, P., and Brewer, C. F. (1...

objective(s) the immunoadjuvant potential of cross-linked dextran microspheres (cdm) as absorption enhancer and quillaja saponins (qs) as immunomodulator adjuvant was evaluated. materials and methods cdm loaded or tetanus-mixed toxoid (tt) or quillaja saponin (qs) were nasally administered to rabbits in dry powder form, three times in 2 weeks interval and serum igg and nasal lavage siga titers ...