AIM To generate human embryonic stem cell derived corneal endothelial cells (hESC-CECs) for transplantation in patients with corneal endothelial dystrophies. MATERIALS AND METHODS Feeder-free hESC-CECs were generated by a directed differentiation protocol. hESC-CECs were characterized by morphology, expression of corneal endothelial markers, and microarray analysis of gene expression. RESUL...

BACKGROUND To present a method, alternative to penetrating keratoplasty, for the restoration of impaired corneal clarity with anterior stromal scarring following long-standing corneal graft failure. CASE PRESENTATION A 48-year old female who had previously underwent Descemet stripping automated endothelial keratoplasty (DSAEK) for the treatment of pseudophakic bullous keratopathy, presented w...

neovascularization of the normally avascular cornea was associated with a notable increase in the expression of the major pro-angiogenic factors, inflammatory cytokines and proteases. the data supporting a causal role for vascular endothelial growth factor (vegf), inflammatory cytokines and matrix metalloproteinase (mmps) are extensive. anti-angiogenic/anti-inflammatory therapy is considered as...

Abstract Background Cataract surgery is traumatic to the corneal epithelium,scarring and opacity commonest cause of blindness. Objective To study epithelial thickness in different conditions using anterior segment optical coherence tomography (AS-OCT). Patients Methods A case-control including 80 eyes divided equally into 4 groups; group 1:controls,group 2:corneal scarring ,group 3:cataract pat...

Purpose The purpose of this study was to clarify correlations between corneal deformation parameters measured with a dynamic Scheimpflug analyzer and baseline factors such as axial length, intraocular pressure (IOP), age, central corneal thickness (CCT), and corneal curvature. Methods Ninety-six eyes of 96 healthy subjects (mean 55.2 ± 16.1 years of age) were examined using a dynamic Scheimpf...

Connective Tissue Growth Factor (CTGF) and Transforming growth factor-β1 (TGF-β1) are key growth factors in regulating corneal scarring. Although CTGF was induced by TGF-β1 and mediated many of fibroproliferative effects of TGF-β1, the signaling pathway for CTGF production in corneal scarring remains to be clarified. In the present study, we firstly investigated the effects of c-Jun N-terminal ...

PURPOSE Amino-amide or amino-ester local anesthetics, which are currently used for topical ocular anesthesia, are short acting and may delay corneal healing with long-term use. In contrast, site 1 sodium channel blockers (S1SCBs) are potent local anesthetics with minimal adverse tissue reaction. In this study, we examined topical local anesthesia with two S1SCBs, tetrodotoxin (TTX) or saxitoxin...