Improving umbilical cord blood stem cell engraftment by ex vivo expansion of hematopoietic stem and progenitor cells 1. Impaired thymopoiesis is associated with severe infections after double umbilical cord blood transplantation (dUCBT). Monitoring of sjTREC + T cell recovery in dUCBT patients should therefore be considered to identify patients in need for intensified support. (this thesis) 2. ...

stem cells are self-renewing and undifferentiated cell types that can be differentiate into functional cells. stem cells can be classified into two main types based on their source of origin: embryonic and adult stem cells. stem cells also classified based on the range of differentiation potentials into totipotent, pluripotent, multipotent, and unipotent. multipotent stem cells have the ability...

Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high e...

Background: There is evidence that mesangial cell structural changes contribute to the pathogenesis of diabetic nephropathy. To gain better insight into the mechanisms responsible for this issue, present study focused on effect of cord blood mononuclear cells (MNCs) derived CD133 positive cells on mesangial cell structure and function. Materials and Methods: The animals were randomly divided in...

We analyzed the factors and outcome of patients with disseminated adenovirus infection (dAdV) after allogeneic hematopoeitic stem cell transplantation (HSCT). Thirty patients with dAdV were identified among 620 allogeneic HSCT recipients. Primary diseases were leukemia (n=17), Fanconi anemia (n=12) or others (n=1). Source of stem cells was unrelated in 28 and related in 2 patients. The graft co...

OBJECTIVE We describe the relation between the clamping time and blood volume collected, plus two enrichment systems for CD34+ stem cells from umbilical cord blood, to achieve an excellent recovery of cells with a high proliferative ability and bone marrow reconstitution. METHOD After cord blood collection by an obstetrician, stem cell purification has been performed either with a combination...

background nowadays using the stem cells has opened a new horizon in the medical science and is indeed a practical and non-invasive method for treating refractory diseases such as leukemias , lymphomas, blood disorders and so on. nonetheless, proper and sustainable uses of such powerful cells undoubtedly need to be as per certain ethical principles. of the existing challenges in using stem cell...

Since the events of ionizing radiation damage increasingly, the human healthy are threatened by radiation. With the development of cells regeneration, stem cells applied in therapy for radiation injury become a hotspot in recent years. Then we are aimed to study on experimental animals to human umbilical cord blood stem cells of ionizing radiation-induced hematopoietic injury. The 80 mice were ...

There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal benefits including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency o...