With the aid of a novel poly-dA tailing-partial restriction technique and S1-protection mapping, the 5' terminal coding sequence for the 40S precursor ribosomal RNA of Xenopus laevis has been exactly identified. Since the promoter sequence for the 40S RNA should lie close to its 5' terminal coding sequence, we are able to conclude that the "Bam-Island" sequence reduplication (1) almost certainl...

Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC). Chromatograms are fitted by Exponentially Modified Gaussian functions (EMG) and mapping of band broadening is obtained for different column sets. Interpretation of the skewing of the chromatogra...

The Santa Barbara post-wildfire debris flows and the Brumadinho tailing-dam failure were two of most catastrophic flood events late 2010s. Both these carried so much solid-phase material, that classic, clear-water, risk approaches cannot replicate them, or forecast other like them. This case study applied new non-Newtonian features in HEC-RAS 6.1 to events, testing widely used model on common m...

Abstract Reliable assessment of earthen dams’ stability and tailing storage facilities widely used in the mining industry is challenging, particularly under seismic load conditions. In this paper, we propose to take into account effect dominant frequency on tailing/earthen dams. The calculations are performed by finite element modelling (FEM) with Mohr–Coulomb failure criteria. To separate cont...

The RTH flotation tailing dump, located in the old open pit Bor about 500 m southeast of Flotation Plant, has been continuous operational work since 1985. flotations dumps are important mining facilities that constantly changed during their exploitation period. Due to changes occurring dump its exploitation, it is necessary perform a technical and technological surveillance-auscultation order c...

In flies, 22-23-nucleotide (nt) microRNA duplexes typically contain mismatches and begin with uridine, so they bind Argonaute1 (Ago1), whereas 21-nt siRNA duplexes are perfectly paired and begin with cytidine, promoting their loading into Ago2. A subset of Drosophila endogenous siRNAs-the hairpin-derived hp-esiRNAs-are born as mismatched duplexes that often begin with uridine. These would be pr...

Gold extraction operations generate a variety of wastes requiring responsible disposal in compliance with current environmental regulations. During recent decades, increased emphasis has been placed on effluent control and treatment, in order to avoid the threat to the environment posed by toxic constituents. In many modern gold mining and ore processing operations, cyanide species are of most ...