نتایج جستجو برای: copper e coli

تعداد نتایج: 1201406  

ژورنال: :مجله دانشگاه علوم پزشکی کردستان 0
محمد یوسف علیخانی mohammad yousef alikhani microbiology dept., faculty of medicine, hamadan, iranدانشکده پزشکی، گروه میکروبشناسی، همدان، ایران محمد مهدی اصلانی mohammad mehdi aslani mcrobiology dept., pasteur institute of iran, hamadan, iranگروه میکروب شناسی، انستیتو پاستور ایران، همدان، ایران علی صادق ali sadegh master of microbiology dept., faculty of medicine, hamadan, iranکارشناس ارشد میکروب شناسی، دانشکده پزشکی، گروه میکروبشناسی، همدان، ایران

زمینه و هدف: سویه های اشریشیاکلی تولیدکننده شیگاتوکسین (stec) بعنوان یکی از عوامل ایجاد بیماریهای اسهالی شناخته شده اند. توانائی کلونیزاسیون این سویه ها در روده انسان جزء ضروری از عفونت ناشی از این باکتری ها محسوب می گردد. به منظور تعیین اهمیت فاکتور چسبندگی در سروتیپ های n‏on-o157 stec ایزوله شده از بیماران مبتلا به اسهال و بدون علائم گوارشی، الگوی چسبندگی این سروتیپ ها به سلولهای hela مورد بر...

ژورنال: :فیض 0
مرتضی صادقی morteza sadegi genetic research center, baqiyatallah university of medical sciences, tehran, i. r. iran.تهران، میدان ونک، خیابان ملاصدرا، دانشگاه علوم پزشکی بقیه الله، بخش ژنتیک زهره حجتی zohreh hojjati

سابقه و هدف: سنتز پروتئین­های نوترکیب با خاصیت مهار گیرنده­ های رشد در تومورهای سرطانی یکی از راه­ های جدید درمان سرطان است. هدف مطالعه حاضر طراحی و کلون ایزوفرم نوترکیبvegf111b  در وکتور pbudce4.1 و بررسی سازگاری این سازه با  باکتری e. coli top10  جهت تولید داروی نوترکیب است.  مواد و روش ­ها: ایزوفرم جدید vegf111b با استفاده از توالی­های موجود در بانک­های ژنی و نرم­افزار 7  oligoطراحی شده و تو...

Journal: :Microbiology 2005
Christopher J Kershaw Nigel L Brown Chrystala Constantinidou Mala D Patel Jon L Hobman

The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - پژوهشگاه ملی مهندسی ژنتیک وزیست فناوری 1390

چکیده فارسی: در این تحقیق ژن دفنسین(rs-afp1) گیاه تربچه با استفاده از pcr از ناقل puc19 جداسازی و سپس در ناقل بیانی pet26b(+) کلون گردید. صحت کلون شدن ژن در ناقل با استفاده از colony pcr وهضم آنزیمی مورد تأیید قرار گرفت و ناقل نوترکیب حاصله pet26rs1 نام گذاری گردید. ناقل pet26rs1به باکتری e. coli bl21(de3) plyss منتقل گردید و با iptg 1میلی مولار و در دماهای 37 و28 درجه سانتیگراد القاءء گردید. ...

Journal: :jundishapur journal of microbiology 0
masoumeh navidinia department of medical bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran shahin najar peerayeh department of medical bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran; department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran. tel/fax: +98-2182883870 fatemeh fallah pediatric infection research centre, mofid children hospital, shahid beheshti university of medical sciences tehran, ir iran bita bakhshi department of medical bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran

results we found a high number of pai markers such as pai icft073, pai iicft073, pai i536, pai iv536, pai ii j96 and pai ii536 significantly associated with upec. the pai iv536 was the most detected ones among fecal e. coli isolated from healthy people while pai i536, pai ii536 and pai ij96 were not seen. pcr phylogenetic groups typing revealed that uropathogenic strains were mainly found in b2...

Journal: :journal of health sciences and surveillance system 0
mahtab hadadi department of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran yalda malekzadegan department of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran hamid heidari department of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran hadi sedigh ebrahim-saraie department of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran mohammad motamedifar department of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran shiraz hiv/aids research center, shiraz university of medical sciences, shiraz, iran

abstract background: escherichia coli, known as a clinically significant bacteria, can cause a wide range of infections, including urinary tract infections (utis), blood stream infections (bsis), and can frequently be isolated from various clinical specimens. evaluation of antimicrobial resistant pattern is a necessary action, especially about such bacteria which are frequent and life threateni...

Journal: :iranian journal of microbiology 0
maryam farahmandfar naghmeh moori-bakhtiari department of microbiology, faculty of veterinary medicine, shahid chamran university of ahvaz, iran saad gooraninezhad 2department of clinical sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, iran mehdi zarei 3department of food hygiene, faculty of veterinary medicine, shahid chamran university of ahvaz, iran

background and objectives: the most common serotype of enterohaemorrhagic esherichia coli group or shiga-toxin-producing e. coli is o157:h7. domestic and wild ruminants are regarded as the main natural reservoirs. o157:h7 serotype is the major cause of gastrointestinal infections in developed countries. in this study was conducted to survey on the toxigenic e. coli o157: h7 strains in milk of i...

The aim of this study was to assess the prevalence of ESBL-producing E. coli in agricultural fields of Gorgan district, Golestan province. Soil samples were collected from 20 agricultural fields, half of them treated with chemical fertilizers and the rest with bovine manure. Two samples, from surface and a depth of 15cm, were taken. After enrichment and culture onto MacConkey agar, Pink colored...

Alisha Akya Mehrdad Khodadoost

Background and Aims: The rate of urinary tract infection caused by the extended-spectrum-β-lactamase- (ESBL) producing Escherichia coli (E. coli) is increasing worldwide. This study aimed to assess the frequency of blaCTX-M genes in the E. coli isolated from urinary tract infection (UTI) in outpatients. Methods: 240 E. coli bacteria were isolated from the outpatients’ urine samples in the Kerma...

D.W. Lukman, D.Y. Sari, H. Pisestyani,

Background: Kebab is one of the popular ready-to-eat foods in Indonesia and sold as a street food. The purpose of this study was to determine occurrence and antibiotic resistance of Escherichia coli in street-vended kebab in Dramaga, Bogor, Indonesia. Methods: Totally, 43 samples of kebab meat were collected from street food kebab vendors. Examinations on Total Plate Count (TPC), and total E. ...

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