A new gene encoding the Hc domain of Clostridium botulinum neurotoxin serotype F (FHc) was designed and completely synthesized with oligonucleotides. A soluble recombinant Hc of C. botulinum neurotoxin serotype F was highly expressed in Escherichia coli with this synthetic FHc gene. Subsequently, the purified FHc was used to vaccinate mice and evaluate their survival against challenge with acti...

to evaluate the effect of probiotic (primalac) and salmonella enteritidis-specific igy on prevention of salmonella enteritidis infection in broiler chickens, four 33 week-old single comb white leghorn hens were hyperimmunized with salmonella enteritidis (se) whole cell antigens obtained by ultrasonication and administrated at a protein concentration of 500 ?g/ml after centrifugation. primary im...

Abstract We identified the lowest composition necessary to generate a vaccination adjuvant that promotes Treg response following immunization in mice and named it “complete tolerogenic adjuvant.” This novel may allow us utilize well-established “antigen plus adjuvant” method of induce cell-mediated antigen-specific immunosuppression. The minimal is dexamethasone, rapamycin, monophosphoryl lipid...

calcium mobilization is known to be an important factor in the activation of cells involved in inflammation,so,calcium-channel antagonists are be expected ti exhibit antiinflammatory activity.in the present study,we evaluateted the antiinflammatory effects of two calcium channel blockers,verapamil and nifedipine on adjuvant induced chronic in flammation in rat paw.sixty adult male rats were div...