Female BALB/c mice were used for immunization with peptide-KLH conjugate. The first immunization was performed using Freund’s complete adjuvant. Incomplete Freund’s adjuvant was used for the second, third, fourth, and fifth immunizations. Finally, 3 days before cell fusion, KLH-peptide (without adjuvant) was injected intravenously. Mice with higher titers of specific antibody were selected for ...