نتایج جستجو برای: colorimetric determination
تعداد نتایج: 259274 فیلتر نتایج به سال:
The activity of carbonic anhydrase has been estimated by three procedures: manometric (l-3), calorimetric (2, 4-8), and electrometric (9). For investigations of the enzyme concentration of tissues the calorimetric method has often been used in preference to the manometric technique because of its relative simplicity. A disadvantage of the calorimetric method has been the inhibitory effects of t...
In connection with the study of the state of the calcium of the blood, we desired a rapid method for determining serum proteins. Wu (1) and Wu and Ling (2) have published a method for the determination of plasma proteins based on the color developed with Folin’s phenol reagent. The method as described by these authors is not very satisfactory due to the formation of turbid precipitates and the ...
All heptoses produce with orcinol in dilute HCl two characteristic colored compounds which differ greatly in their absorption spectra. Three different modifications of this reaction were found useful. Procedure i--To 2 cc. of a solution containing 20 to 100 y per cc. of ketoheptose or 5 times as much of an aldoheptose is added 0.4 cc. of concentrated HCl, sp. gr. 1.19, in a test-tube with a gro...
An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. The test is carried out with 1 ml of urine, which is processed through a 10-mm-d...
A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-nitrophenol, which can be quantified by measuring the absorption at 400 nm in neutral or basic media. The reactions proceed fast under basic conditions and complete in 2 minutes to...
SINcE the publication of von Fellenberg's method [1923] for the determination of small quantities of iodine, a very large number of new methods and modifications have been described, some of which claim a surprising degree of accuracy. The multiplicity of methods is sufficient to show that none of them is really satisfactory. The von Fellenberg method includes two methods for the final determin...
A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizu...
A method is described for the colorimetric determination of ammonia in plasma by a batch ion-exchange procedure. The method combines separation of ammonium ion, using a strongly acidic cation-exchange resin, with a sensitive colorimetric procedure which simultaneously elutes and colorimetrically reacts with the ammonium ion to produce a stable color. Interference by basic amino acids is negligi...
Determination of the 4-hydroxy-l-proline (hydroxyproline) concentration may provide useful information for the diagnosis and prognosis of diseases caused by disorders of collagen metabolism. The objective of the present study was to apply liquid chromatography-mass spectrometry (LC-MS) to assess the hydroxyproline concentration. The hydroxyproline concentration in lung and liver tissues measure...
The Tauber perchioric acid method and the Fischl colorimetric procedure were combined into a quantitative method for tryptophan that is applicable directly to proteins without hydrolysis. The colorimetric tryptophan method was compared with both salt fractionation and electrophoretic methods for 26 normal sera and those from 51 patients with various chronic diseases. Analysis of the results for...
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