There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in the assay of biopharmaceuticals. The techniq...

A new approach to general sensors for odors and volatile organic compounds (VOCs) using thin films of chemically responsive dyes as a colorimetric sensor array is described. This optoelectronic “nose,” by using an array of multiple dyes whose colors change based on the full range of intermolecular interactions, provides enormous discriminatory power among odorants in a simple device that can be...

All heptoses produce with orcinol in dilute HCl two characteristic colored compounds which differ greatly in their absorption spectra. Three different modifications of this reaction were found useful. Procedure i--To 2 cc. of a solution containing 20 to 100 y per cc. of ketoheptose or 5 times as much of an aldoheptose is added 0.4 cc. of concentrated HCl, sp. gr. 1.19, in a test-tube with a gro...

A comparison of two colorimetric procedures for the determination of serum glutamic oxaloacetic acid (SCOT) showed that both the dinitrophenylhydrazine and diazonium salt colorimetric assay procedures have good precision and reproducibility acceptable to most clinical laboratories. Babson’s diazonium salt procedure (3) definitely shows greater precision in the important borderline range of valu...

Colorimetric quantification of p-aminohippurate (PAH), which is helpful in assessment of renal function, is subject to interference from sulfa compounds such as sulfamethoxazole. We were unable to measure serum PAH in two patients receiving a trimethoprim-sulfamethoxazole combination because of interference by the latter with the colorimetric estimation. We found that isoamyl acetate removes su...

Hue plane preserving color correction (HPPCC), introduced by Andersen and Hardeberg [Proceedings of the 13th Color and Imaging Conference (CIC) (2005), pp. 141-146], maps device-dependent color values (RGB) to colorimetric color values (XYZ) using a set of linear transforms, realized by white point preserving 3×3 matrices, where each transform is learned and applied in a subregion of color spac...

Gold nanoparticles functionalized with thiolated oligonucleotides (Au-nanoprobes) have been used in a range of applications for the detection of bioanalytes of interest, from ions to proteins and DNA targets. These detection strategies are based on the unique optical properties of gold nanoparticles, in particular, the intense color that is subject to modulation by modification of the medium di...

One of the main factors limiting agricultural production in tropical climate regions is mainly related to the presence of exchangeable aluminum (Al) in highly weathered acid soils. Four methods of Al determination extracted with neutral 1 mol L KCl solution were evaluated: three colorimetric methods (aluminon plus ascorbic acid, and eriochrome cyanine R by FIA) and the usual titrimetric method ...

(1) Background: d-alanine-d-alanine ligase (DdlA), an effective target for drug development to combat against Mycobacterium tuberculosis (Mtb), which threatens human health globally, supplies a substrate of d-alanyl-d-alanine for peptidoglycan crosslinking by catalyzing the dimerization of two d-alanines. To obtain a better understanding of DdlA profiles and develop a colorimetric assay for hig...

Although of the many colorimetric microestimations of sugar the Folin and Wu method (1920) has remained in most widespread use in clinical laboratory practice, the still increasing number of modifications continue to draw attention to several inherent weaknesses of the method which have not yet been eliminated, one of which is the instability of the colour solution. To avoid these drawbacks a m...