In two hamster cell lines that differed 100-fold in their vinblastine sensitivity, dissolution of the mitotic spindle by vinblastine in living cells correlated with cytotoxicity from vinblastine expressed as suppression of colony formation. The effect on the spindle apparatus occurred in 30 sec or less and thus provides a rapid assay for determining the cytotoxic effects of the Vinca alkaloids,...

Purpose: To elucidate the role of AKAP12 in leukemia cells.Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting (WB) were employed to determine expression leukocyte cell lines, while 5-azacytidine was used treat cells, followed by assessment AKAP12. After constructing overexpressing vector pc-AKAP12 transfecting it into cells or treating with 5-az...

Abstract Background The long noncoding RNA gastric cancer associated transcript 3 (GACAT3) has been demonstrated to be implicated in the carcinogenesis and progression of many malignancies. However, GACAT3’s levels role esophageal squamous cell carcinoma (ESCC) not elucidated. Methods GACAT3 amounts were investigated ESCC tissues lines by qPCR. Its biological functions examined CCK-8 assay, col...

BACKGROUND The aim of this study was to investigate and interpret the expression level and potential function of TCEA3 in gastric cancer. MATERIAL AND METHODS qRT-PCR was used to determine the expression level of TCEA3 in gastric cancer tissues. Pearson χ2 test was performed to clarify the correlation between TCEA3 expression and patients' clinicopathologic characteristics. Biological functio...

Abstract A large number of researches have shown that circular RNA (circRNA) is new hope for the diagnosis or treatment tumors, including liver cancer (LCa). However, it remains largely unclear role circRNA in progression LCa and its molecular mechanism. GSE164803 microarray dataset was applied to identify dysregulated circRNAs noncancerous tissues. CircTUBGCP5 (hsa_circ_0034049) selected furth...

Introduction: 10Z-Himenialdicin is a natural product derived from the marine sponge Axinella carteri. It has reported that an anti-angiogenetic effect through NF-κB which produces angiogenic factors such as VEGF and IL-8. Although there no previous report revealed effects of on cancer cells, potential inhibiting prevent metastasis in pancreatic cancer. Method: We investigated cell lines followi...

We studied the cell kill induced by granulocyte-macrophage colony-stimulating factor (GM-CSF ) fused to Diphtheria Toxin (DT-GM-CSF ) in acute myeloid leukemia (AML) samples and in populations of normal primitive hemopoietic progenitor cells. AML samples from three patients were incubated in vitro with 100 ng/mL DT-GM-CSF for 48 hours, and AML cell kill was determined in a proliferation assay, ...

BACKGROUND FBG2 (F-BOX6) gene is an important member in ubiquitin metabolic system F-BOX family, and forms E3 complex with the other members in the family. But its role in gastric cancer is still not clear. In the present study, we intended to investigate the influence of FBG2 on the growth, proliferation, apoptosis, invasion and cell cycle of the gastric cancer line MKN45 and gastric cell line...

Objectives. Chemotherapy is considered to be essential in the treatment of patients with colorectal cancer (CRC), but drug resistance reduces its efficacy. Many advanced CRC eventually show 5-fluorouracil (5-FU) therapy. Synergistic and potentiating effects combination therapy, using herbal chemical drugs, can improve patients’ response. Zerumbone (ZER), which derived from ginger, has been stud...