BACKGROUND One of the most fascinating properties of the biotechnologically important organism Saccharomyces cerevisiae is its ability to perform simultaneous respiration and fermentation at high growth rate even under fully aerobic conditions. In the present work, this Crabtree effect called phenomenon was investigated in detail by comparative 13C metabolic flux analysis of S. cerevisiae growi...

Tangshen formula (TSF), a well-prescribed traditional Chinese formula, has been used in the treatment of diabetic nephropathy. However, whether TSF ameliorates dyslipidemia and liver injury associated with diabetes remains unclear. In this study, we examined the effects of TSF on lipid profiles and hepatic steatosis in db/db mice. For this purpose, 8‑week-old db/db mice were treated with TSF or...

OBJECTIVE Malonyl coenzyme A (CoA) decarboxylase (MCD) is a key enzyme responsible for malonyl-CoA turnover and functions in the control of the balance between lipid and glucose metabolism. We utilized RNA interference (siRNA)-based gene silencing to determine the direct role of MCD on metabolic responses in primary human skeletal muscle. RESEARCH DESIGN AND METHODS We used siRNA to silence M...

Background: In recent decades, aortic stenting has become a promising alternative to surgery for both native coarctation and re-stenosis in children adults. However, comparative long-term outcomes have poorly been investigated. Methods: We included 212 patients with previous repair (19 ± 8.7 years) divided into 3 groups: 139 single-time surgical (CoA-S group); 18 percutaneous (CoA-PS 55 hybrid ...

the coarctation of the aorta (coa) is rare in adulthood. diagnosis is made by clinical suspicion and physical findings such as blood pressure difference between the upper and lower extremities, pulse delay in the femoral artery, and systolic murmur over the thoracic spine.the coa in adulthood and in patients with associated aneurysm is challenging and different complications even with proper tr...

gamma-amino butyric acid (gaba) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. production of gaba-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. in this study, glutamate decarboxylase (gad) gene of a loca...

In primary cultures of adult rat hepatocytes maintained in a salts/glucose medium, a more than 100-fold increase in ornithine decarboxylase (EC 4.1.1.17) activity was caused by asparagine and glucagon in a synergistic manner. The synthesis rate of ornithine decarboxylase was determined by [35S]methionine incorporation into the enzyme protein, and the amount of ornithine decarboxylase-mRNA was m...