In contrast to the classical world, an unknown quantum state cannot be cloned ideally, as stated by the no-cloning theorem. However, it is expected that approximate or probabilistic quantum cloning will be necessary for different applications, and thus various quantum cloning machines have been designed. Phase quantum cloning is of particular interest because it can be used to attack the Bennet...

The efficiency of PCR product cloning depends on the nature of the DNA polymerase employed because amplicons may have blunt-ends or 3' adenosines overhangs. Therefore, for amplicon cloning, available commercial vectors are either blunt-ended or have a single 3' overhanging thymidine. The aim of this work was to offer in a single vector the ability to clone both types of PCR products. For that p...

We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisation reaction. The single-stranded overhangs are designed to allow directional cloning in a ligase-free manner. This 'enzyme-free cloning'...

Many new reproductive methods such as artificial insemination, in vitro fertilisation, freezing of human embryos, and surrogate motherhood were at first widely condemned but are now seen in Western society as not just ethically and morally acceptable, but beneficial in that they allow otherwise infertile couples to have children. The idea of human cloning was also quickly condemned but debate i...

While the no-cloning theorem, which forbids the perfect copying of quantum states, is well-known as one of the defining features of quantum mechanics, the question of how well the theory allows a state to be cloned is yet to be completely solved. In this paper, rigorous solutions to the problem of M → N asymmetric cloning of qudits are obtained in a number of interesting cases. The central resu...

We have constructed two plasmid vectors for cloning and expression of DNA fragments controlled by the lac promoter as a NotI-expression cassette. Whereas plasmid pSJ33 allows mobilization of the expression cassette into a wide variety of Gram-negative bacteria by RP4-mediated conjugation, the low-copy-number plasmid pSJP18Not facilitates cloning and expression in Escherichia coli when high gene...

The safety of quantum cryptography relies on the no-cloning theorem. In secret quantum communications, an eavesdropper cannot clone the sent qubits perfectly, however the best eavesdropping attacks for quantum cryptography are based on imperfect cloning machines. The eavesdropper’s physically allowed quantum evolutions on the sent qubit can be described in terms of the quantum state’s geometry....

As an increasing number of genes and open reading frames of unknown function are discovered, expression of the encoded proteins is critical toward establishing function. Accordingly, there is an increased need for highly efficient, high-fidelity methods for directional cloning. Among the available methods, site-specific recombination-based cloning techniques, which eliminate the use of restrict...

The relative error of cloning of states with arbitrary prior probabilities is considered. It is assumed that ancilla may contain some a priori information about states to be cloned. The lower bound on the relative error for general cloning scenario is derived. Both the case of two-state set and case of multi-state set are analyzed. The made conclusions complete subject of the stronger no-clonin...

Recent advances in genomics and biotechnology have ushered in a new era in health development. Therapeutic cloning possesses enormous potential for revolutionizing medical and therapeutic techniques. Cloning technology, however, is perceived as having the potential for reproductive cloning, which raises serious ethical and moral concerns. It is important that the Islamic countries come to a con...