Fanconi anemia (FA) patients have an exacerbated risk of head and neck squamous cell carcinoma (HNSCC). Treatment is challenging as FA display enhanced toxicity to standard treatments, including radio/chemotherapy. Therefore, better therapies well new disease models are urgently needed. We used CRISPR/Cas9 editing tools in order interrupt the human FANCA gene by generation insertions/deletions ...

Bullous pemphigoid (BP) is an autoimmune blistering disorder induced by autoantibodies against type XVII collagen (COL17). COL17-reactive CD4+ T cells have been shown to play important role in the pathogenesis of BP. However, characteristics pathogenic remain unclear. In this study, we sought establish cell clones and elucidate their pathogenicity. First, immunized wild-type mice with human COL...

Background: IFN-g is mostly secreted by activated CD4+ , CD8+ T cells and NK cells. This cytokine has immunomodulatory, anti-cancer and anti-microbial effects and is important for prophylaxis, diagnosis and treatment of chronic infections and cancers. Objective: The purpose of this study was to clone the full cDNA of human IFN-g and express it in CHO cell line. Methods: Lymphocytes from a healt...

Background: Monoclonal antibodies (mAbs) are essential tools for many molecular im-munology investigations, epitope mapping and molecular modelling, clinical laboratory di-agnostic tests and immunotherapy. Humoral immune response of immunized animals largely depends on the nature of antigen and the immunization technique. Polysaccharides and heavily-glycosylated proteins are very elusive target...

Objective(s): In the current research, the prevalence of Staphylococcus aureus clones and genes encoding antimicrobial resistance and toxins were examined among 120 S. aureus strains from nosocomial infections in tehran, Iran.Materials and Methods: Antimicrobial susceptibility was examined, based on disk diffusion and PCR method to identify resistance and toxin-encoding genes. Based on the poly...