We have screened a human DNA library using as probe a chimpanzee sequence that contains homology to the polymerase gene of the endogenous baboon virus. One set of overlapping clones spans about 20 kilobases and contains regions of DNA sequence homology to the gag p30, gag p15, and polymerase genes of Moloney murine leukemia virus. Furthermore, the spacings are the same as in Moloney virus betwe...

We read with great interest and appreciation the article by Weis and Levy 1 and the editorial by Koppel 2 in a recent issue of CHEST (March 2014). If we may make additional observations about the day-to-day review of records from those of us that perform health information reviews, there are more problems. One of us is on the faculty at National Jewish Health and consults on many out-of-state p...

Sheep, mice, cattle, goats and pigs have all been cloned by transfer of a donor cell nucleus into an enucleated ovum, and now we add the successful cloning of a cat (Felis domesticus) to this list. However, this cloning technology may not be readily extendable to other mammalian species if our understanding of their reproductive processes is limited or if there are species-specific obstacles.

data. Although there were some differences they concluded: “Because onset and end dates of the growing season were averaged over the entire Tibetan Plateau, the temporal resolution of the data was deemed sufficiently high for detailed analysis.” So they tested whether winter warming might affect the plants’ chilling requirements in this cold, highaltitude environment. They determined the beginn...

We studied the extracellular [HCO ] dependence of two renal clones of the electrogenic Na/HCO 3 cotransporter (NBC) heterologously expressed in Xenopus oocytes. We used microelectrodes to measure the change in membrane potential ( D V m ) elicited by the NBC cloned from the kidney of the salamander Ambystoma tigrinum (akNBC) and by the NBC cloned from the kidney of rat (rkNBC). We used a two-el...

The number of species for which somatic cell nuclear transfer (SCNT) protocols are established is still increasing. Due to the high number of cloned farm, companion, and sport animals, the topic of animal cloning never ceases to be of public interest. Numerous studies cover the health status of SCNT-derived animals, but very few cover the effects of SCNT on aging. However, only cloned animals t...

One of the most widely used analytical techniques for sensitive detection of biologically and clinically significant analytes is the immunoassay. In recent years direct immunoprobes allowing label-free detection of the interaction between the antibody and the target analyte have proved their capabilities as fast, simple, and nevertheless highly sensitive methods. Cloned enzyme donor immunoassay...

A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.

Essentially all plant species exhibit heritable genetic variation for resistance to a variety of plant diseases caused by fungi, bacteria, oomycetes or viruses. Disease losses in crop monocultures are already significant, and would be greater but for applications of disease-controlling agrichemicals. For sustainable intensification of crop production, we argue that disease control should as far...

1 Pyrenean Institute of Ecology (IPE), Spanish National Research Council (CSIC), 22700 Jaca, Spain. Tel.: (+34) 976 369 393. E-mail: [email protected] 2 Department of Animal Production (Division of Wildlife), Faculty of Life Sciences and Engineering, University of Lleida, 25198 Lleida, Spain. Tel.: (+34) 973 003 721 Division of Conservation Biology, Institute of Ecology and Evolution. Univer...