P. aeruginosa M14808 was screened to produce rhamnolipid with high yield by mutation in a high magneto-gravitational environment. After silica gel columniation chromatogram, the components of the crude biosurfactant extract were analyzed by TLC, FTIR, and ESI-MS. The major components were characterized as rhamnolipids. The rhamnolipids consisted of two main types, monoand di-rhamnolipids. Di-rh...

Uremic ultrafiltrates (and normal serum, for comparison) were fractionated by means of gel filtration. The collected fractions were further investigated by combined analytical techniques: "high-performance" liquid chromatography, gas chromatography, mass spectrometry, and isotachophoresis. Ultrafiltrate fractions in the so-called middle molecular mass region (Mr 500-2000) contained a considerab...

Barbiturates are extracted quantitatively into ethyl ether from biologic fluids buffered to pH 6. A measured portion of the ether extract is then impinged to dryness and the residue spotted on Whatman No. 2 paper that has been previously dipped in alkali and dried. The paper is developed by descending chromatography with chloroform saturated with water. The barbiturate may then be located and a...

A four-step strategy is described which is aimed at optimizing the separation parameters of the chromatographic fingerprint of herbal medicines. In the optimization procedure, a model is built to predict the retention time of components. The proposed model shows good retention prediction potency with an average relative deviation of 1.78%. The grid search method is used in optimizing gradient p...

Upon base composition analysis, oligonucleotides which are labeled at the 3'-terminus with fluorescein or biotin generate an additional, late eluting peak in the HPLC chromatogram. Investigation of this effect revealed the haptens acted as apurinic sites, and phosphodiesterase cleavage of the phosphate bond between the upstream nucleotide and apurinic site is inhibited. Extension of this work w...

A new reversed-phase high performance liquid chromatography method was developed to quantitate the activity of xanthine oxidase involved in milk fat globule membrane with xanthine as the substrate and the separation of product (uric acid). The increment of uric acid in the reaction system was used to calculate the total activity of XO. The optimized assay conditions, linearity of detection, rec...

This is a review article concernning the development of a gas chromatographic analytical method for the analysis of 70 air toxic compounds. Not only the operating conditions, but also the column system is optimized. Optimization of the GC analytical methods for air toxic compounds is based on a unified method with a system comparising two columns. An optimization strategy with stepwise temperat...

In this paper, neural network technique is applied to a system named NEUCOMS that is designed for condition monitoring of power transformers. Through employing paired neural networks, NEUCOMS has the ability of analyzing data of electrical inspections as well as data of the chromatogram of oil-dissolved gases. It utilizes redundant input attributes to speed the training and reduce the size of t...

Wense has reported the isolation of crystalline adrenalin from larvae of the beetle Tenebrio molitor. An attempt to repeat his procedures failed to yield any evidence of adrenalin. Analysis of mealworm extracts by paper chromatography and colorimetric means also yielded no indication of adrenalin, though adrenalin added to mealworms can be detected by these procedures in amounts less than 1 mg....

The aim of this paper is to develop a rapid, and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. ⅰ)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolideⅠ, pac...