In this study, the complexity of chromatin integrity was investigated in frozen-thawed semen samples from 37 sires with contrasting fertility, expressed as 56-day non-return rates (NR56). Protamine deficiency, thiols, and disulfide bonds were assessed compared previously published data for DNA fragmentation index (DFI) high stainability (HDS). addition, vitro embryo development sperm methylatio...

BACKGROUND Checkpoint signaling pathways are of crucial importance for the maintenance of genomic integrity. Within these pathways, the effector kinase Chk1 plays a central role in mediating cell-cycle arrest in response to DNA damage, and it does so by phosphorylating key cell-cycle regulators. RESULTS By investigating the subcellular distribution of Chk1 by cell fractionation, we observed t...

Significance CENP-A, the histone H3 variant that forms a unique centromeric chromatin, is essential for faithful chromosome segregation during mitosis. Inability to connect centromere mitotic spindle causes aneuploidy, hallmark of many cancers. In addition missegregation, fusions at (peri)centromeres are prevalent in cancers, but how such rearrangements arise remains unclear. Here, we identifie...