Lamins, the major structural components of the nuclear lamina (NL) found beneath the nuclear envelope, are known to interact with most of the nuclear peripheral chromatin in metazoan cells. Although NL-chromatin associations correlate with a repressive chromatin state, the role of lamins in tethering chromatin to NL and how such tether influences gene expression have remained challenging to dec...

DNA damage signaling and repair machineries operate in a nuclear environment where DNA is wrapped around histone proteins and packaged into chromatin. Understanding how chromatin structure is restored together with the DNA sequence during DNA damage repair has been a topic of intense research. Indeed, chromatin integrity is central to cell functions and identity. However, chromatin shows remark...

The structure of [3H]thymidine pulse-labeled chromatin in lymphocytes differs from that of non-replicating chromatin by several operational criteria which are related to the higher nuclease sensitivity of replicating chromatin. These structural features of replicating chromatin rapidly disappear when the [3H]thymidine pulse is followed by a chase in the presence of an excess of non-radioactive ...

Chromatin structures have to be precisely duplicated during DNA replication to maintain tissue-specific gene expression patterns and specialized domains, such as the centromeres. Chromatin remodeling factors are key components involved in this process and include histone chaperones, histone modifying enzymes and ATP-dependent chromatin remodeling complexes. Several of these factors interact dir...

The ChromDB website (http://www.chromdb.org) displays chromatin-associated proteins, including RNAi-associated proteins, for a broad range of organisms. Our primary focus is to display sets of highly curated plant genes predicted to encode proteins associated with chromatin remodeling. Our intent is to make this intensively curated sequence information available to the research and teaching com...

Chromatin fibers composed of DNA and proteins fold into consecutive loops to form rod-shaped chromosome in mitosis. Although the loop growth dynamics is investigated several studies, its detailed processes are unclear. Here, we describe time evolution length for thermal-driven as an iterative map by calculating physical quantities involved processes. We quantify energy during chromatin formatio...

Abstract Cells are often under attack from various DNA-damaging agents. Accurate repair is required to protect cells the genome instability induced by DNA lesions. damage response (DDR) signaling involves sensitizing, transmitting, and repairing different types of within chromatin complexes. Chromatin a highly ordered complex packed with repeating units nucleosomes linker sequences. structure, ...

Abstract CD8+ T cells provide a critical layer of host protection against all manner pathogens and malignancies. Chromatin remodeling is component differentiation acquisition cytotoxic functions in activated cells, but the mechanisms by which achieved site-specific way to promote appropriate effector cell fates are poorly understood. Here we identified role for ARID1A-containing canonical BAF (...

Packing of the eukaryotic genome into chromatin poses an accessibility problem for the DNA repair machinery. Chromatin structure has to be changed for the repair to occur, and we are beginning to discover how different chromatin modifying mechanisms facilitate DNA repair in the chromatin context. On the other hand, the repair-related changes in chromatin should be transient, and a particular ch...

Rat liver chromatin has been fractionated by different solubility in solvents of 155 mM ionic strength in soluble S and insoluble I-chromatin. Histone H1 content is lower in S as compared to I-chromatin. The HMG1/2 nonhistone proteins are observed in S-chromatin and in the nuclear pelleted residue from the chromatin isolation procedure, but no amount can be detected in I-chromatin. Thermal dena...