In this study we established human vaginal epithelial cells (hVECs) in culture and evaluated their interaction with Trichomonas vaginalis parasites to complement previous studies using other cell types. Primary cultures of hVECs were established. Contaminating fibroblasts were separated from epithelial cells by differential trypsinization. Specific antibody staining revealed that over 92% of ce...

The clinical significance, Gram stain reaction, and genus affiliation of Gardnerella vaginalis have been controversial since Gardner and Dukes described the organism as the cause of "nonspecific vaginitis," a common disease of women which is now called bacterial vaginosis. The organism was named G. vaginalis when taxonomic studies showed that it was unrelated to bacteria in various genera inclu...

BACKGROUND Trichomonas vaginalis is a flagellated protozoan causing trichomoniasis, a sexually transmitted human infection, with around 276.4 million new cases estimated by World Health Organization. Culture is the gold standard method for the diagnosis of T. vaginalis infection. Recently, immunochromatographic assays as well as PCR assays for the detection of T. vaginalis antigen or DNA, respe...

Trichomonas vaginalis is" the microbial agent responsible for trichomonal vaginitis, a major sexually transmitted disease throughout the world (1-4). The complex events involved in trichomonad parasitism of host cells are poorly understood. We have focused on the ability of T. vaginaiis to selectively acquire host-derived macromolecules in order to better define host-trichomonad interactions (5...

Both Mycoplasma hominis and Trichomonas vaginalis utilize arginine as an energy source via the arginine dihydrolase (ADH) pathway. It has been previously demonstrated that M. hominis forms a stable intracellular relationship with T. vaginalis; hence, in this study we examined the interaction of two localized ADH pathways by comparing T. vaginalis strain SS22 with the laboratory-generated T. vag...

BACKGROUND In spite of sufficient knowledge about phenotypic variation of Trichomonas vaginalis, its genetic characteristics are poorly understood. We carried out a molecular epidemiology study in which in vitro metronidazole susceptibility of T. vaginalis isolates was considered. METHODS This study was conducted on 862 women admitted to Gynecology Clinics in Hamadan, west of Iran, during 201...

Gardnerella vaginalis strain JCM 11026(T) was isolated from vaginal tracts of women. Here, we report the complete genome sequence of this organism.

In order to reduce the psychological and aesthetic consequences caused by elective or therapeutic orchiectomy, we developed two types of testicular prostheses, which were produced using a 3D printer, with polylactic acid filament, coated cyanoacrylate polymethylmethacrylate (PMMA). They tested in Wistar rats, orchiectomy performed on left testicle prosthesis implanted inside tunica vaginalis, g...