The photosystem I reaction center complex, the P-700-chlorophyll a-protein, has been isolated from the photosynthetic membranes of two marine dinoflagellates, Gonyaulax polyedra and Glenodinium sp., by detergent solubilization with Triton X-100. The complexes isolated from the two species were indistinguishable, exhibiting identical absorption properties (400-700 nm) at both room (300 K) and lo...

1. The fluorescence spectra of the alga Porphyridium have been recorded as energy distribution curves for eleven different incident wave lengths of monochromatic incident light between wave lengths 405 and 546 mmicro. 2. In these spectra chlorophyll fluorescence predominates when the incident light is in the blue part of the spectrum which is strongly absorbed by chlorophyll. 3. For blue-green ...

The disappearance of chlorophyll is a visual sign of fruit ripening. Yet, chlorophyll breakdown in fruit has hardly been explored; its non-green degradation products are largely unknown. Here we report the analysis and structure elucidation of colorless tetrapyrrolic chlorophyll breakdown products in commercially available, ripening bananas (Musa acuminata, Cavendish cultivar). In banana peels,...

Changes in the functional organization of chlorophyll occur during light-dependent chlorophyll accumulation in yellow mutants of Chlamydomonas reinhardti£ These changes were studied using single-photon counting techniques to determine the chlorophyll fluorescence decay kinetics at various times after transfer of de-greened cultures to the light. Several different yellow mutants were analyzed: y...

NITRIC OXIDE-ASSOCIATED1 (NOA1) encodes a circularly permuted GTPase (cGTPase) known to be essential for ribosome assembly in plants. While the reduced chlorophyll and Rubisco phenotypes were formerly noticed in both NOA1-suppressed rice and Arabidopsis, a detailed insight is still necessary. In this study, by using RNAi transgenic rice, we further demonstrate that NOA1 functions in a temperatu...

nickel and cadmium are heavy metals with some hazards for plant metabolism. the impacts of nickel chloride (0, 400 and 800 μm) in the presence of vitamin b complex (0, 30 and 60 ml from pharmacy commercial stock) and the impacts of cadmium chloride (0, 100, 200, 300, 400, 500 and 600 μl from 100 µm stock) in pot and cadmium nitrate (0, 100, 200, 400, 800, 1600 and 3200 μl from 100 µm stock) in ...

The techniques utilized to extract chlorophyll from plant materials are destructive and based on methods that use organic solvents. This study proposes the non-destructive quantification of chlorophyll and nitrogen concentration using a portable chlorophyll meter (SPAD-502). Further, was possible to establish relationships between chlorophyll fluorescence and SPAD-502 values. This methodology c...

Chlorophyll is a key indicator of the physiological status of a forest canopy. However, its distribution may vary greatly in time and space, so that the estimation of chlorophyll content of canopies or branches by extrapolation from leaf values obtained by destructive sampling is labor intensive and potentially inaccurate. Chlorophyll content is related positively to the point of maximum slope ...

The development of plastid membranes was studied in relation to chlorophyll accumulation in dark-grown barley seedlings of various ages after transfer to light. Quantitative electron microscopy showed that the prolamellar body membranes are reorganized into primary lamellar layers which contain sufficient membranes to support grana formation during 24 h of greening. Structural reorganization of...