This protocol describes a technique for high-resolution chloride imaging of living cells using a quinoline-based chloride (Cl(-)) indicator dye, MQAE (N-[6-methoxyquinolyl] acetoethyl ester). Bath-applied to acute brain slices, MQAE provides high-quality labeling of neuronal cells and their processes. In living anesthetized mice, cortical cells are labeled using the multicell bolus loading proc...

  Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study.   Materials and Methods: White Leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in D...

A kinetic investigation of the effect of sodium chloride on cell growth of Lactobacillus amylovorus DCE 471 and amylovorin L471 production was carried out through in vitro experiments using a temperature and pH prevailing during sourdough fermentations. Sodium chloride interfered both with cell growth and bacteriocin production. Biomass formation and amylovorin L471 production decreased in the ...

Cystic Fibrosis (CF) is the most common lethal monogenic disorder in Caucasians, estimated to affect one per 2500-4000 newborns. CF is caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) [1, 2]. CFTR acts mainly as a chloride channel and has other regulatory roles, including inhibition of sodium transport through the epithelial sodium channel, regulation o...

This paper describes a new method for the recovery of high-concentration ammonia from water in form ammonium chloride, hydroxide and carbonate. The was applied to Solvay process, which sodium bicarbonate is produced through reaction ammoniated brine CO2 gas. effluent contains soluble chloride. proposed based on using high-alkalinity reactant, calcium oxide (CaO), closed electrocoagulation cell ...

The present study was designed to observe the properties of swelling-activated chloride channel (ICl.swell) in mouse cardiac myocytes using patch clamp techniques. In whole-cell recordings, hypotonic solution activated a chloride current that exhibited outward rectification, weak voltage-dependent inactivation, and anion selectivity with permeability sequence of I- > Br- > Cl-. The current was ...

This historical approach to past CF research shows that the evidence for the mucus hydration view is slender and can be called into question as providing adequate proof. This approach also indicates that associated smooth muscle cell pathophysiology in CF has been overlooked but may prove to be the more productive hypothesis. Present views on the pathophysiology of cystic ibrotic lung function ...

Chloride-induced corrosion of steel reinforcement is a major factor adversely influencing the durability of reinforced concrete structures. The main objective of this preliminary study was to explore the use of waste residues generated during the bioethanol processing of sugar cane bagasse as a source of high value products, primarily lignin that can be functionalized to scavenge chloride ions ...

A MORPHOLOGICAL BASIS FOR OSMOREGULATION IN THE TELEOSTS WAS STUDIED BY COMPARING THE FINE STRUCTURE OF CHLORIDE CELLS FOUND IN EPITHELIA OF THE GILLS OF THREE SPECIES OF FISH: Fundulus heteroclitus which can survive in a wide range of salinities, and F. similis and F. chrysotus which are usually restricted to salt water and fresh water environments, respectively. Gills were removed from F. het...

Human thymocytes and peripheral blood lymphocytes were separated on the basis of density on Percoll gradients and the different cell populations tested regarding DNA synthesis after addition of mercuric chloride. Thymocytes with a density of 1.065-1.067 g/ml and peripheral blood lymphocytes with a density of 1.063-1.065 g/ml were maximally stimulated with mercuric chloride as well as with mitog...