A patient with infectious mononucleosis complicated by acute haemolytic anaemia is described. The patient had, in addition to non-specific cold agglutinins in the serum, a positive Donath-Landsteiner reaction in the absence of syphilis. The association of a positive Donath-Landsteiner reaction with haemolytic anaemia in infectious mononucleosis has been described only once before.

Coronavirus defective RNAs (D-RNAs) have been used for site-directed mutagenesis of coronavirus genomes and for expression of heterologous genes. D-RNA CD-61 derived from the avian coronavirus infectious bronchitis virus (IBV) was used as an RNA vector for the expression of chicken gamma interferon (chIFN-gamma). D-RNAs expressing chIFN-gamma were shown to be capable of rescue, replication, and...

viral infections are the cause of great economic losses in the poultry industry. development of appropriate reagents and serological diagnostic kits will help to control these infections. the aim of this study is to prepare a peroxidase labeled anti-chicken igg using a mab (5b8) against chicken igg, for detection of chicken antibodies in elisa. hybridoma cells producing the mab 5b8 were culture...

TT virus (TTV) is widespread among the global population. Its pathogenic nature is still unclear but TTV seems to be more prevalent in cases of hepatitis than in healthy individuals. TTV harbours similarities to chicken anaemia virus (CAV). Here, the apoptotic potential of a putative TTV-derived 105 aa protein and of the main apoptosis-inducing agent of CAV, Apoptin, is compared. As the putativ...

Since the demonstration of the transmissibility of chicken tumors by agents separable from the malignant cells, the relationship of this group of neoplasms to those of the mammalian type has been a question of first importance. At first it was generally considered that the chicken tumor agents belonged to the virus group of organisms. This conclusion led to some doubt that the chicken tumors re...

The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cu...

chicken infectious bronchitis virus (ibv) causes severe respiratory and renal dysfunction in chickens. in the present study, we used reverse transcription-polymerase chain reaction (rt-pcr) to amplify the nucleocapsid (n) protein gene of strain massachusetts (mass) h120 of ibv that is commonly used in the vaccine production in iran. the pcr product with the expected size of 1.2 kb was cloned in...

Infectious bursal disease virus (IBDV) isolates were recovered from outbreaks to initiate activities towards developing a local vaccine strain. Use of indigenous chicken embryos was exploited to determine their potential, promote utilization of local resources for research, and enhance household economic activities. Bursa of Fabricius (BFs) samples from outbreaks shown to be IBDV positive was h...

INTRODUCTION Persons with HIV infection frequently present with anaemia from different causes, including use of antiretroviral therapy (typically zidovudine), iron deficiency, vitamin B12 deficiency, opportunistic infections (such as mycobacterial and fungal infections), chronic disease, AIDS-associated malignancies, autoimmune haemolysis, and direct effects of HIV infection itself. Persistent ...