Bacillus Calmette-Guërin(BCG) stimulated the proliferation of granulocyte-macrophagecolony-formingcells (CFU) and activated suppressor cells that inhibit the immunization of T lymphocytes in vitro. Increases in both CFU concentration and suppressor cell activity were moderate in the bone marrow and marked in the spleen of mice given BCG i.v. In the bone marrow, these increases were apparent 2 ...

Hematopoiesis is thought to be modulated by interactions of progenitor cells with hematopoietic growth factors. We have shown that colony-forming units-spleen (CFU-S) and repopulating stem cells require interleukin-3 (IL-3) to survive in vitro, and that CFU-S number and long-term repopulating ability can be increased by culture in the combination of IL-3 and IL-6. In this report, we describe th...

Myeloflbrosis with myeloid metaplasia (MMM) is a chronic myeloproliferative disorder due to clonal expansion of a pluripotent hematopoietic progenitor cell with secondary marrow fibrosis. No definitive treatment has as yet been devised for this condition, which shows a marked variability in clinical course. To evaluate whether excessive hematopoietic progenitor cell proliferation could be contr...

The erythroid colon-forming technique has been used to study the erythropoietin (Ep) sensitivity of CFU-E during ontogenesis. Evidence is provided which indicates that an age-related decreasing Ep sensitivity of CFU-E occurs as the animal develops. In addition, an intermediate Ep-sensitive CFU-E population represented by the 2-day-old neonate is present during the transition from foetus to adul...

We analyzed fosfomycin susceptibility results in Pseudomonas aeruginosa clinical isolates obtained by MIC gradient strips and disk diffusion methods using two different inocula, 10(8) and 10(6) CFU/ml, and compared them to the agar dilution reference method. Essential and categorical agreements were 93.6% and 95%, respectively, for the 10(6) CFU/ml alternative inoculum, and they were 67.6% and ...

We have identified a molecule expressed by human marrow granulocyte/monocyte colony-forming cells (CFU-GM), erythroid colony-forming cells (CFU-E), and erythroid burst-forming units (BFU-E), but not their precursors detectable in long-term bone marrow culture. This antigen, detected by flow microfluorimetry using monoclonal antibody 7B9, is coexpressed with CD33 on many CD34+ CFCs, although onl...