An immunoconjugate, consisting of both toxin and radionuclide on the same antibody molecule, was synthesized by cross-linking the phytotoxin ricin to the T101 monoclonal antibody recognizing the CD5 cluster expressed on normal and malignant T-cells. The hybrid molecule was then labeled with iodine-125 by an iodine monochloride procedure. This radioimmunotoxin (RIT), which selectively bound to t...

Deacylcortivazol (DAC), a potent glucocorticoid, contains a phenyl-pyrazole moiety fused to the 2--3 position of the traditional steroid nucleus. When incubated with glucocorticoid-resistant mutants derived from the glucocorticoid-sensitive human leukemic cell line CEM-C7, DAC caused significant growth inhibition. However, this effect required 1 microM DAC, a concentration 50 times higher than ...

OBJECTIVES To investigate the efficacy of interleukin 11 (IL-11) towards the high dose methotrexate (HDMTX)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human T-lymphoblastic leukemia (CEM) cell line. MATERIALS AND METHODS 95 Wistar rats were randomly divided into five groups, the normal control group (A), the methotrexate (MTX) control group (B), the ...

We aimed to determine the metabolic profile and effects of chemotherapy on ovarian cancer cell metabolism in a three-dimensional (3D) vs. a two-dimensional (2D) construct using 31P-magnetic resonance spectroscopy (MRS). Three ovarian cancer cell lines were embedded in a 3D perfused Matrigel construct or grown in a 2D monolayer. Metabolic differenc...

background: pattern recognition receptors (prrs) are the main sensors of pathogen and danger signals in innate immunity of which toll like receptors (tlrs) are the most studied ones. the contribution of prrs in cerebral inflammation induced by microbial infection, tissue damage and cancer has not extensively been addressed so far. glioma is the most common tumor of the central nervous system an...

background: interfering with cell proliferation and survival is a critical role for antineoplastic drugs leading to cell death through induction of apoptosis. alternative treatments with herbal extracts offer insights into acute myeloid leukemia (aml) therapy. parthenolide (ptl), an extract from feverfew, induces apoptosis in primary human leukemia stem cells (lscs) and bulk leukemic cell popul...

Laxaphycin A (laxa A) and Laxaphycin B (laxa B), cyclic peptides isolated from the terrestrial blue-green alga Anabaena laxa or the marine cyanobacterium Lyngbya majuscula have antifungal and cytotoxic activities. We used numerical videomicrofluorometry and a protocol of multiple labelling with Hoescht 33342 (nuclear DNA), Rhodamine 123 (mitochondria) and Nile Red (plasma membrane) to study the...