The chimeric proteins viz. CBM3-Cel9A, CBM4-Cel9A and CBM30-Cel9A, are constructed by fusion of family 3, 4, and 30 cellulose binding modules (CBMs) to N-terminus of family 9 endoglucanase (Cel9A) from Alicyclobacillus acidocaldrious. The chimeric enzymes were successfully expressed in Escherichia coli and purified to homogeneity. The chimeric enzymes showed significant increase in Avicel (8-12...

Sustainable transportation biofuels may require considerable changes in land use to meet mandated targets. Understanding the possible impact of di erent policies on land use and greenhouse gas emissions has typically proceeded by exploring either ecosystem or economic modelling. Here we integrate such models to assess the potential for the US Renewable Fuel Standard to reduce greenhouse gas emi...

BACKGROUND For the development of lignocellulosic biofuels a common strategy to release hemicellulosic sugars and enhance the enzymatic digestibility of cellulose is the heat pretreatment of biomass with dilute acid. During this process, fermentation inhibitors such as 5-hydroxymethylfurfural, furfural, phenolics, and organic acids are formed and released into the so-called hydrolysate. The phe...

Corn cob hydrolysate was used as substrate for growth and lipid accumulation via oleaginous yeast species. A mass based suspension of 10 g 100 g corn cob hydrolysate contained 26.0 g L glucose, 8.5 g L xylose. The inhibitor concentrations were 0.16 g L acetic acid, 1.50 g L formic acid, 0.48 g L HMF and 0.06 g L furfural. These conditions reduced the cell growth of non-adapted yeast. Successful...

OBJECTIVE To compare Pressure Ulcer Scale for Healing (PUSH) scores at 8 weeks in long-term-care residents with pressure ulcers who were given standard care plus a concentrated, fortified, collagen protein hydrolysate supplement vs. residents who were given standard care plus placebo. DESIGN Randomized, prospective, controlled, multicenter trial at 23 long-term-care facilities in 4 states. ...

0306-2619/$ see front matter 2011 Elsevier Ltd. A doi:10.1016/j.apenergy.2011.08.020 ⇑ Corresponding author. Tel.: +351 253604424; fax E-mail addresses: [email protected], so (S.I. Mussatto). Significant amounts of wastes are generated by the coffee industry, among of which, coffee silverskin (CS) and spent coffee grounds (SCG) are the most abundantly generated during the beans roasting and...

In order to get products with antioxidant activity from Arca subcrenata Lischke, the optimal hydrolase and hydrolysis conditions were investigated in the paper. Three proteases (neutrase, alcalase and papain) were applied to hydrolyze the homogenate of A. subcrenata. An orthogonal design was used to optimize hydrolysis conditions, and the pH-stat methods was used to determine the degree of hydr...

The effects of adding mechanically deboned chicken (MDC) hydrolysates on the quality characteristics of imitation crab stick (ICS) during storage were investigated. ICS was prepared from Alaska Pollack, chicken breast surimi, and protein hydrolysates enzymatically extracted from MDC. ICS samples were divided into 4 groups: without protein hydrolysate (control), added with 0.5% protein hydrolysa...

In this research scotch pine (Pinus sylvestris L.), Eastern beech (Fagus orientelis L.), oak (Quercus petraea L.) samples have been covered with polyurethane, acrylic, cellulosic varnish. In order to determine the physical changes which occurred on varnish layers caused by steam and wet heat, the samples were exposed to TS 4982 EN 12721 wet heat and TS EN 438-2 steam. According the results, the...

In order to use sugarcane bagasse as a substrate for lactic acid production, optimum conditions for acid hydrolysis of the bagasse were investigated. After lignin extraction, the conditions were varied in terms of hydrochloric (HCl) or sulfuric (H(2)SO(4)) concentration (0.5-5%, v/v), reaction time (1-5h) and incubation temperature (90-120 degrees C). The maximum catalytic efficiency (E) was 10...