Abstract In-cell NMR, i.e., NMR spectroscopy applied to studying specific macromolecules within living cells, is becoming the technique of choice for structural and mechanistic description proteins nucleic acids increasingly complex cellular environments, as well temporal evolution biological systems over a broad range timescales. Furthermore, in-cell has already shown its potentialities in ear...

ABSTRACT Fertilization triggers significant cellular remodeling through the oocyte-to-embryo transition. In this transition, ubiquitin-proteasome system and autophagy are essential for degradation of maternal components; however, significance cell surface components remains unknown. study, we show that multiple plasma membrane proteins, such as glycine transporter GlyT1a, selectively internaliz...

Genes encoding proteins involved in the early steps of protein glycosylation are dysregulated yeast RNA exosome mutants through mechanisms that involve increased accumulation noncoding RNAs, leading to cell wall instability.

Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...

Objective(s):Small ubiquitin-like modifiers (SUMOs) are a family of ubiquitin-related, proteins that are involved in a wide variety of signaling pathways. SUMOylation, as a vital post translational modification, regulate protein function in manycellular processes. Diazinon (DZN), an organophosphate insecticide, causses oxidative stress and subsequently programmed cell death in different tissues...

plant growth is a result of cell division and cell enlargement. expansins are proteins that mediate long-term extension of isolated cell walls and they play a variety of roles in plant cells. in this study the atexpb2 gene was over-expressed in arabidopsis thaliana. the gene was isolated from genomics dna of arabidopsis and cloned into mediated vectors; pgemt and pbi121 vectors. transformation ...

protein secretory systems are found in all living organisms including gram negative bacteria, and eukaryotic cell’s organelles that are derived from these bacteria. unlike all other living organisms, gram negative bacteria have different protein secretory systems. at least there are four different independent secretory systems, which are responsible to transport the secreted proteins to the mil...

In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...

Background: Some resources have suggested that genetically inactivated pertussis toxoid (PTs) bear a more protective effect than chemically inactivated products. This study aimed to produce new version of PT, by cloning an inactive pertussis toxin S1 subunit (PTS1) in a fusion form with N-terminal half of the listeriolysin O (LLO) pore-forming toxin. Methods: Deposited pdb structure file of the...