Arachidonic acid (AA) and Gamma linolenic acid have been shown to limit glioma cell growth, stimulate apoptosis and lipid peroxidation. However, brain tumours are characterised by cellular heterogeneity and responding cell populations have not been identified. Brain tumour samples from patients were disaggregated. In cell preparations from 7 gliomas, reactive oxygen species (ROS), morphology an...

Mahadevan, P. R. (The Rockefeller Institute, New York, N.Y.), and E. L. Tatum. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J. Bacteriol. 90:1073-1081. 1965.-The relationship of cell wall to morphology in Neurospora crassa was studied by correlating the levels of structural polymers of the cell wall with wild-type and colonial m...

The behaviour in culture of epithelial cells derived from chick embryo pigmented retina epithelium (PRE), corneal epithelium (CE) and epidermis has been studied using time-lapse cinemicrography. The analysis concentrated on the morphology and movement of individual isolated cells, lacking contacts with other cells, during a 24h period starting 1-3 h after the cells were plated out in primary cu...

Hydrogels prepared from a bacterial fimbriae are utilized for the 2D cell culture of human dermal fibroblasts. The tunable biochemical and mechanical nature Caf1 hydrogels allow modulation morphology matrix deposition.

Test compounds may have undesired side affects in addition to the desired modulation of a specific signaling pathway. Here we describe the use of high content cell-based assays to monitor such effects. In addition to the primary activity readout, many image analysis algorithms used for high content analysis report secondary readouts describing the cellular morphology, including number of cells,...

Changes in the cell and nuclear morphology of sparsely plated WI38 fibroblasts were followed as a function of time after increasing the serum concentration from 0.3% to 10%. Quantitative measurements were carried out in parallel on Feulgen-stained nuclei and Wright-stained cells using the Quantimet 720-D image analysis system. We report a rapid, significant change in nuclear morphology indicati...

The corneal endothelium of 42 clear corneal transplants was studied with a specular microscope. The endothelial cell morphology was analysed by using a computerised image analysis system. A quantitative index was developed to study the degree of variation in cell size. By applying this objective index we observed that pleomorphism is independent of age, and considerable alteration occurs in cel...

Vascular endothelial cell migration is critical in many physiological processes including wound healing and stent endothelialization. To determine how preexisting cell morphology influences cell migration under fluid shear stress, endothelial cells were preset in an elongated morphology on micropatterned substrates, and unidirectional shear stress was applied either parallel or perpendicular to...