asymmetrically mixed matrix matrimid-mil-53 membranes with silicone cover layer were fabricated. for better understanding of membrane fabrication process, three main parameters of fabrication, matrimid concentration, silicone concentration and weight percentage of metal organic framework (mil-53) particles, were optimized by an experimental design method. cross-section sem images were used to s...

Peptides and proteins, two important classes of biomacromolecules, play roles in the biopharmaceuticals field. As compared with traditional drugs based on small molecules, peptide- protein-based offer several advantages, although most cannot traverse cell membrane, a natural barrier that prevents biomacromolecules from directly entering cells. However, drug delivery via cell-penetrating peptide...

Cyclosporin A (CyA) and L-carnitine (LC) prevented the killing of cultured hepatocytes by anoxia and rotenone but not by cyanide. Neither CyA nor LC affected the rate or extent of the loss of the mitochondrial membrane potential or the rate or extent of the depletion of ATP. Atractyloside blocked the ability of both CyA and LC to protect, and D-carnitine antagonized the effect of LC but not tha...

in this research, the bioinsising impact of two wood decay fungi (pysisporinus vitreus and xylaria longipes) on radial air permeability and moisture diffusion coefficient of spruce wood (picea abies) was investigated. fungal cultivation was performed in an incubator at 25 °c and 85% relative humidity for 15, 30 and 45 days. results showed that the permeability, moisture diffusion and mass loss ...

Traditional Transwell inserts with track-etched 10 ?m thick polymer membranes have been intensively used for studying cellular barriers. However, their thickness hampers direct cell-cell interaction between the adjacent cells which has shown to critically influence barrier formation. Therefore, here effect of reduced distance by using fivefold thinner (2 ?m) optically transparent polydimethylsi...

Polyglycerol monolaurates are generally recognized as safe food additives and commonly used emulsifiers. In this study, the antimicrobial effect of four polyglycerol on two Gram-positive bacteria (Staphylococcus aureus Bacillus subtilis) Gram-negative (Escherichia. coli Pseudomonas aeruginosa) were investigated. The minimum inhibitory concentration (MIC) diglycerol monolaurate (PG2ML), triglyce...

In human liver, Ca(2+)-dependent changes in membrane K(+) permeability play a central role in coordinating functional interactions between membrane transport, metabolism, and cell volume. On the basis of the observation that K(+) conductance is partially sensitive to the bee venom toxin apamin, we aimed to assess whether small-conductance Ca(2+)-sensitive K(+) (SK(Ca)) channels are expressed en...

Coupled cellular respiration requires that ATP and ADP be efficiently exchanged between the cytosol and the mitochondrial matrix. When growth factors are withdrawn from dependent cells, metabolism is disrupted by a defect in ATP/ADP exchange across the mitochondrial membranes. Unexpectedly, we find that this defect results from loss of outer mitochondrial membrane permeability to metabolic anio...

Nanosecond, high-voltage electric pulses (nsEP) induce permeabilization of the plasma membrane and the membranes of cell organelles, leading to various responses in cells including cytochrome c release from mitochondria and caspase activation associated with apoptosis. We report here evidence for nsEP-induced permeabilization of mitochondrial membranes in living cells. Using three different met...

Graphene materials can emit far-infrared ray, but the biological effects of graphene ray have not been studied. Furthermore, non-thermal effect on organism has systematically studied independently thermal effect. The purpose this study was to investigate (gFIR) Saccharomyces cerevisiae cells. In work, stringent control cultivation conditions carried out ensure stability and constancy culture it...