The distribution of glycine decarboxylase (GDC) in leaves of pea (Pisum sativum L.) and wheat (Triticum aestivum L.) has been investigated using immunogold labeling of the P-protein subunit of the GDC complex. Mitochondria in photosynthetic mesophyll cells were densely labeled, whereas those in nonphotosynthetic vascular parenchyma and epidermal cells were only weakly labeled. In pea leaves the...

Studying protein components of large intracellular complexes by in-cell NMR has so far been impossible because the backbone resonances are unobservable due to their slow tumbling rates. We describe a methodology that overcomes this difficulty through selective labeling of methyl groups, which possess more favorable relaxation behavior. Comparison of different in-cell labeling schemes with three...

A rapid and efficient approach for preparing isotopically labeled recombinant proteins is presented. The method is demonstrated for 13C labeling of the C-terminal domain of angiopoietin-2, 15N labeling of ubiquitin and for 2H/13C/15N labeling of the Escherichia coli outer-membrane lipoprotein Lpp-56. The production method generates cell mass using unlabeled rich media followed by exchange into ...

‎a $(p‎,‎q)$ graph $g$ is said to have a $k$-odd mean‎ ‎labeling $(k ge 1)$ if there exists an injection $f‎ : ‎v‎ ‎to {0‎, ‎1‎, ‎2‎, ‎ldots‎, ‎2k‎ + ‎2q‎ - ‎3}$ such that the‎ ‎induced map $f^*$ defined on $e$ by $f^*(uv) =‎ ‎leftlceil frac{f(u)+f(v)}{2}rightrceil$ is a‎ ‎bijection from $e$ to ${2k - ‎‎‎1‎, ‎2k‎ + ‎1‎, ‎2k‎ + ‎3‎, ‎ldots‎, ‎2‎ ‎k‎ + ‎2q‎ - ‎3}$‎. ‎a graph that admits $k$...

The discrete Hamiltonian of Su, Schrieffer and Heeger (SSH) is a well-known one-dimensional translation-invariant model in condensed matter physics. consists two atoms per unit cell describes in-cell out-of-cell electron-hopping between sub-lattices. It among the simplest models exhibiting non-trivial topological phase; to SSH one can associate winding number, Zak phase, which depends on ratio ...

Cell labeling and tracking are becoming increasingly important areas within the field of stem cell transplantation. The ability to track the migration and distribution of implanted cells is critical to understanding the beneficial effects and mechanisms of stem cell therapy. The present study investigated the effects of amine-surface-modified superparamagnetic iron oxide (SPIO) nanoparticles on...

When estimating the labeling index is of interest, the design of experiments raises a number of methodological questions: How many cells should be scored? How big a difference in labeling index is likely to be detectable? What is the potential effect of low growth fraction on detecting a treatment effect? What are appropriate ways of expressing treatment effects on labeling index? Data from two...

We report a highly specific, robust and rapid new method for labeling cell surface proteins with biophysical probes. The method uses the Escherichia coli enzyme biotin ligase (BirA), which sequence-specifically ligates biotin to a 15-amino-acid acceptor peptide (AP). We report that BirA also accepts a ketone isostere of biotin as a cofactor, ligating this probe to the AP with similar kinetics a...

Let $G$ be a graph. Let $f:V(G)to{0,1,2, ldots, k-1}$ be a map where $k in mathbb{N}$ and $k>1$. For each edge $uv$, assign the label $left|f(u)-f(v)right|$. $f$ is called a $k$-total difference cordial labeling of $G$ if $left|t_{df}(i)-t_{df}(j)right|leq 1$, $i,j in {0,1,2, ldots, k-1}$ where $t_{df}(x)$ denotes the total number of vertices and the edges labeled with $x$.A graph with admits a...

Glutamine synthetase was localized in nodules, roots, stems, and leaves of red kidney bean (Phaseolus vulgaris L.) by immunocytochemistry. Affinity purified antibodies reactive with glutamine synthetase were prepared using purified nodule-enhanced glutamine synthetase. Immunogold labeling was observed in the cell cytoplasm in each plant organ. In nodules, the labeling was more intense in the in...