THE usual graphical method of expressing the increase of bacterial growth rate in a media by means of the bacterial number/time curve, has several disadvantages, inasmuch as that the maximum growth rate cannot be accurately determined, nor the period of optimum propagation. An improved method of presenting a bacterial growth-rate curve and eliminating the aforementioned disadvantages will now b...

A Synechococcus sp. PCC 7942 bioreporter strain capable of sensing bioavailable Fe was constructed by fusing the Fe-responsive isiAB promoter to the Vibrio harveyi luxAB genes. Monitoring luxAB-dependent luminescence through the growth curve demonstrated that in Fe-replete media, transcription from the isiAB promoter was induced transiently in the mid-exponential phase of growth. The initiation...

background: the current study investigated the possible role of the cell cycle regulator, cyclin-dependent kinases regulatory subunit-2; the angiogenic factor, plateletderived endothelial cell growth factor; and the cell adhesive molecule, neural cadherin, as prognostic factors in schistosomal and non-schistosomal-associated urothelial carcinomas. we also investigated the possible correlation b...

Cultured skin fibroblast cells were prepared from two patients with mucolipidosis III (ML III), which is a genetic disorder characterized by low activities of multiple lysosomal enzymes in fibroblasts. Genetic complementation analysis of fused fibroblast hybrids revealed that the patients were classified in different complementation groups. Growth curves of fibroblasts of ML III patients in cul...

Ovarian cancer is the most lethal gynecological cancer in which cisplatin-based treatment plays a fundamental role as the first line chemotherapy option. However, development of platinum-resistance is a critical and poorly understood problem in ovarian cancer treatment. Although in vitro generation of platinum-resistant ovarian cancer cell lines is a long established approach to uncover the mol...

Color change of pH indicators in broth medium is commonly used to quantify growth of ureaplasmas. These organisms differ from other members of the Mollicutes by their ability to hydrolyze urea to CO2 and NH3. This study describes a method which continuously monitors color change in ureaplasmal broth cultures. Using this technique we found: (i) there was a pH-dependent absorbance at 554 nm in ur...

OBJECTIVE By date investigations have indicated the presence of stem cells within the pulp tissue of both temporary and permanent human teeth. In the present study, these stem cells were compared in terms of their growth kinetics and culture requirements. MATERIALS AND METHODS Stem cells within the pulp of the human third molar (permanent tooth) and the deciduous incisor (temporary tooth) wer...

introduction: electroporation is a practical technique used to transport the molecules across the cell membrane. the utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. it is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the chang...