The VLA-4 (CD49d/CD29) integrin is the only member of the VLA family expressed by resting lymphoid cells that has been involved in cell-cell adhesive interactions. We here describe the triggering of homotypic cell aggregation of peripheral blood T lymphocytes and myelomonocytic cells by mAbs specific for certain epitopes of the human VLA alpha 4 subunit. This anti-VLA-4-induced cell adhesion is...

Limb bud chondrogenesis in vivo and in vitro appears to depend upon extensive cell contacts leading to localized increases in relative cell density. When cultured in suspension, dissociated early limb bud cells segregate into aggregating and non-aggregating populations. While the non-aggregating cells die, resulting aggregates differentiate exclusively as cartilage. We are investigating the rol...

The encapsulation of cells together with micro-objects in monodispersed water-in-oil microdroplets offers a powerful means to perform quantitative biological studies within large cell populations. In such applications, accurate object detection is crucial ensure control over the content for every compartment. particular, ability rapidly count and localize objects key future applications single-...

Postbiotics are functional bioactive substances manufactured during fermentation in a food matrix, which can be used to improve human health, but their influence on the adhesion potential and physicochemical cell surface of probiotics is still unclear. We examined postbiotic produced by Escherichia coli Nissle 1917 labneh properties (auto-aggregation, hydrophobicity, co-aggregation) capacities ...

Blood is a two-phase suspension of formed elements (i.e., red blood cells [RBCs], white blood cells [WBCs], platelets) suspended in an aqueous solution of organic molecules, proteins, and salts called plasma. The apparent viscosity of blood depends on the existing shear forces (i.e., blood behaves as a non-Newtonian fluid) and is determined by hematocrit, plasma viscosity, RBC aggregation, and ...

Cell aggregation in unicellular organisms, induced by either cell non-sexual adhesion to yield flocs and biofilm, or pheromone-driving sexual conjugation is of great significance in cellular stress response, medicine, and brewing industries. Most current literatures have focused on one form of cell aggregation termed flocculation and its major molecular determinants, the flocculation (FLO) fami...

The aggregative behaviour and subsequent morphogenesis of extra-embryonic endoderm cells from primitive-streak chick embryos have been investigated. A relatively pure population of area opaca endoderm cells was obtained by differential dissociation, which involves partial separation of epiblast and endoderm cell clumps by sieving through Nitex mesh. For aggregation studies cells were cultured i...

Protein aggregation is a hallmark of a growing group of pathologies known as conformational diseases. Their most intensively studied examples include Alzheimer's disease, Parkinson's disease, Huntington's disease and spongiform encephalopathies (prion diseases). Numerous studies have indicated that different polypeptides use common cellular mechanisms to form aggregates. As a consequence, many ...

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