A competitive enzyme-linked immunosorbent assay (cELISA), using two monoclonal antibodies (MAbs), was developed and compared with the standard virus neutralization test (VNT) for detecting antibodies against canine distemper virus (CDV) and phocine distemper virus (PDV) in sera from dogs and various species of marine mammals. The test depends on the blocking of MAb binding to solid-phase antige...

Morbillivirus neutralising antibodies are traditionally measured using either plaque reduction neutralisation tests (PRNTs) or live virus microneutralisation tests (micro-NTs). While both test formats provide a reliable assessment of the strength and specificity of the humoral response, they are restricted by the limited number of viral strains that can be studied and often present significant ...

A growing body of literature has been published indicating that the current practice of annual vaccination of dogs may not be beneficial and in some cases may even be harmful. A number of publications have proposed assessing the immune status of dogs before annual revaccination. In this study the usefulness of a commercially available dot-ELISA kit was evaluated to determine the duration IgG an...

Human cytomegalovirus (CMV) UL54 DNA polymerase (pol) mutants with known patterns of resistance to current antivirals ganciclovir (GCV), foscarnet (FOS), and cidofovir (CDV) were tested for cyclopropavir (CPV) susceptibility by a standardized reporter-based yield reduction assay. Exonuclease and A987G (region V) mutations at codons commonly associated with dual GCV-CDV resistance in clinical is...

Emerging infectious diseases constitute some of the most pressing problems for both human and domestic animal health, and biodiversity conservation. Currently it is not clear whether the removal of past constraints on geographical distribution and transmission possibilities for pathogens alone are sufficient to give rise to novel host-pathogen combinations, or whether pathogen evolution is also...

Mouse hybridomas producing antibodies against structural proteins of canine distemper virus (CDV) were produced by fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with purified preparations of Vero cell-grown CDV. Ascites fluids collected after intraperitoneal inoculation with 149 CDV antibody-producing hybridoma cell lines were characterized by different serological ...

Canine distemper (CD) caused by canine virus (CDV) is considered a highly contagious and acutely febrile disease in various animals around the world. Endoplasmic reticulum-associated protein degradation (ERAD) an important biological effect induced endoplasmic reticulum (ER) stress (ERS) for of unfolded/misfolded proteins ER cells. CDV H glycoprotein translocated into post-translational modific...

Background: Canine distemper virus (CDV) is an extremely contagious pathogen that causes deadly diseases in carnivores worldwide. Objectives: Considering the effect of CDV on host immune system and increased risk other infections, present study aimed to investigate incidence coinfection with Bordetella bronchiseptica, using genomic serological methods. Methods: In this study, 50 blood samples, ...

this study was done to evaluate quality properties of pistacia atlantica var mutica (bene) nuts under deferent storage conditions and packaging. at the zero time, sfa and total tocopherol (tt) and total phenolic (tp) compounds were 6.8 and 725.28 and 40.1 mg per kg oil, respectively. some quality properties (peroxide value (pv), conjugated diene value (cdv) and carbonyle value (cv)) showed that...