Creatine kinase isoenzyme MB catalytic activities in human serum, determined by ACA ion exchange chromatography and immunoinhibition, differ significantly, the correlation coefficient being 0.88. The reasons for this variation are interference of antibodies with the creatine kinase B subunit in the immunoinhibition assay, nonreproducible elution of creatine kinase isoenzyme MB from the ion exch...

Seed-mediated growth of gold nanoparticles on glassy carbon (GC) surfaces was developed. The field emission scanning electron microscopy (FE-SEM) and electrochemical characterization confirmed the effective attachment of gold nanoparticles on GC surface with such a wet-chemical method. The as-prepared gold nanoparticles attached glassy carbon electrode (Au/GCE) presented excellent catalytic abi...

On the basis of the remarkable difference in affinity of graphene (GO) with ssDNA containing a different number of bases in length, we for the first time report a GO-DNAzyme based biosensor for amplified fluorescence "turn-on" detection of Pb(2+). A FAM-labeled DNAzyme-substrate hybrid acted as both a molecular recognition module and signal reporter and GO as a superquencher. By taking advantag...

Dopamine  (DA)  and  uric  acid  (UA)  are  two  of  important bimolecular widely  circulated  in  body blood. Therefore, development of simple and rapid methods for simultaneous determination of them in  routine  analysis  has  a  great  significance  for  many  researchers. Therefore,  for  the  first  time, nanocomposite  of  graphene  (Gr)LaMnO3has  been  utilized  to  fabricate  the  new  ...

Catalase (CAT) is a heme enzyme with a Fe((III/II)) prosthetic group at its redox centre. CAT is present in almost all aerobic living organisms, where it catalyzes the disproportionation of H(2)O(2) into oxygen and water without forming free radicals. In order to study this catalytic mechanism in detail, the direct electrochemistry of CAT has been investigated at various modified electrode surf...

An increased worldwide usage of platinum group elements (PGE) has been observed during recent decades. high amounts of PGE are applied in such areas as chemical industry and jewellery production, but the increased demand for these metals primarily depends on the introduction of automobile catalytic converter systems. Catalytic converters have also been considered to be a major source of PGE pol...

L-Ascorbic acid (AsA) is known for its reductive properties and for its use on a wide scale as an antioxidant agent in foods and drinks; it is also important for therapeutic purposes and biological metabolism. Therefore, recent advances in the food and pharmaceutical industries and the need for nutritional assessments necessitate the development of a selective, simple and accurate method to det...

Innovative materials were made via the combination of chitin and lignin, and the immobilization of lipase from Aspergillus niger. Analysis by techniques including FTIR, XPS and 13C CP MAS NMR confirmed the effective immobilization of the enzyme on the surface of the composite support. The electrokinetic properties of the resulting systems were also determined. Results obtained from elemental an...

Förster resonance energy transfer (FRET) technology has been widely used in biological and biomedical research, and it is a very powerful tool for elucidating protein interactions in either dynamic or steady state. SUMOylation (the process of SUMO [small ubiquitin-like modifier] conjugation to substrates) is an important posttranslational protein modification with critical roles in multiple bio...

1983 is not only the 100th anniversary of the birth of Otto Warburg, but is also the 50th anniversary of the determination of the structure of the flavin chromophore by Kuhn and Wagner-Jauregg (1930) and the 50th anniversary of the isolation of the first flavoprotein, the Old Yellow Enzyme of brewers bottom yeast (Warburg and Christian 1933). In the intervening 50 years, some 200 different flav...